Table 8 Primers used for real time RT-PCR analysis
Gene name | Forward primer | Reverse primer | GenBank Acc. No./TIGR TC |
|---|---|---|---|
Capn1 | 5-GCCAAAACATTGCCTGTTATCTTAG-3 | 5-ATAGGAGGCCGTATCAAAATTCC-3 | AY573919 |
Capn2 | 5-GATTCATCCAGAACGTGTAGG-3 | 5-GGTTAAACACTGGAGCGTGTC-3 | AY573920 |
Cpns | 5-GCTGCCTTCAAATCTGCATGT-3 | 5-TGTACCTGCGAGCGATCAACT-3 | AF482696 |
CAST-S | 5-ATGACAGAGCAGCTGTCCAATC-3 | 5-TGTTGAAGCAACATCACTGCAA-3 | AY937408 |
CAST-L | 5-ACGGCACCTTTCCTTTCCATTACCA-3 | 5-CGGGGGGAGCAGGAGACTTGGT-3 | AY937407 |
Proteasome α5 | 5-GGTGTAGCGCTTCTCTTTGG-3 | 5-ACTGGACAAAGGTGCCTGAT-3 | TIGR database TC78609 |
Proteasome β3 | 5-CCCATGGTGACAGAGGACTT-3 | 5-TGTCTGGCTCCCAGAGAGAT-3 | TIGR database TC87448 |
Proteasome N3 | 5-AAGTGAACGACAGCACCATTC-3 | 5-CCTCATCGATCACCATCTGTT-3 | CA386652 |
Proteasome Regulatory 6 5-CCGACCTCAGAGAAAAGGTG-3 | 5-AGAAGAGGTACTGGCGGACA-3 | TIGR database TC87921 | |
Plyubiqitin | 5-CTGGAAGATGGTCGCACTCT-3 | 5-GATCTGCATACCTCCCCTCA-3 | AF361365 |
Cathepsin-D | 5-GCCTGTCATCACATTCAACCT-3 | 5-CCACTCAGGCAGATGGTCTTA-3 | U90321 |
Cathepsin L | 5-TGAAGGAGAAGATGTGGATGG-3 | 5-TTCCTGTCTTTGGCCATGTAG-3 | AF358668 |
GADPH | 5-CTGAACGACCACTTCGTCAA-3 | 5-TTACTCCTTGGTGGCCATGT-3 | CB491826 |
C3 | 5-CTAACGAGGGCAAGCTCAAC-3 | 5-GCCTCCAGAGTGAGAAGGTG-3 | CB514355 |
ATP5J2 | 5-GGGCGGATAAAAAGGCTAAT-3 | 5-GCCATTTATTGCCTGAAGGA-3 | CB493612 |
ACP5 | 5-CAAGCAGTTCGACTGGATCA-3 | 5-ACCAATGGACCACACAGGAT-3 | CB515428 |
FADS2 | 5-GTCCGTGCTTTGTGTGAGAA-3 | 5-TCAGAGACCCGACAACATCA-3 | CB494661 |
β-Actin | 5-GCCGGCCGCGACCTCACAGACTAC-3 | 5-CGGCCGTGGTGGTGAAGCTGTAAC-3 | AJ438158 |