Table 5 Primers used for RT-qPCR
Contig ID | MIPS ID | Predicted or Known Function | Outside primers (5' to 3') | Inside primers (5' to 3') |
|---|---|---|---|---|
Contig 1438 | -- | -- | F: CGAGCCAAGTGGAGGAAGAAGCGTA | F: TGCGCGCCCGATCCAAGTAAAC |
| Â | Â | Â | R: ACGCTTTAATTTTTTTGGGGCATGTTCT | R: CGCATAGACAAGCAACGACGGTAGGA |
Contig 4572 | UM01426 | Conserved Hypothetical Protein | F: TCCGACTGCAATCTCTACCCGAAACTT | F: GCGCTCGTCAACACAATCATCCATG |
| Â | Â | Â | R: AGGCGAACAGCGGTGGAATCAAC | R: GGGCAAAAGTATCAAGACCAGCATCAGAG |
Contig 4070 | UM00919 | Probably ADP, ATP Carrier Protein | F: CGTCTCGCCGCCCCCTACAA | F: TTCCCTCGGTTGTCGGTATCGTTGT |
| Â | Â | Â | R: CGGCACCCTTGAAGAGCGACG | R: ACACCCTCGGCGGCAATGATG |
Contig 1652 | UM04481 | Related to Alcohol Dehydrogenase II | F: GCTGCCACCATCAAGGACTTCAAAAA | F: CCACCTCAAGCCCGACAAAACATTC |
| Â | Â | Â | R: GACCCTGCAGCTTCTTCGAGTTGTAGTG | R: TCGAAGGGTCCGTCAGCTGGTAAAC |
Contig 2167 | UM04974 | Putative Protein | F: CGCCTGCTGCTGAATCGGACC | F: TCGGGCTGGAGCGTTGATCTGA |
| Â | Â | Â | R: CGGACATGGACAAAGACTCGACGTG | R: GCGCGGTTTGGTCGACATCGT |
Contig 1015 | UM11105 | Nitrate Transporter | F: CGCTCTGCTCATTCTCGGTGCTATTC | F: CGCACTGCCGACAACGAAAAGG |
| Â | Â | Â | R: GGGTGGGTGAGCGGTTGCAGTAA | R: GCGGCGTCGATAGCGGTGAAA |
Contig 4494 | UM01756 | Probable Purine Transporter | F: CGGCTCGCTCGTCGGTTGCT | F: CCCCGCCTTTGTCACCATCGTC |
| Â | Â | Â | R: CGCTCCTCTCGTTCTCCAGTTGCTC | R: CCGCGGGAGAGCTTGAGGATGAT |
Contig 992 | UM03847 | Nitrate Reductase | F: TCGTCGTCATCACCGGCATCCTC | F: GCAAGCCGTTCCTCGATCCCAAG |
| Â | Â | Â | R: TGGCTTCGTTTTCAGGAGTGCAATTG | R: TCTTTGTACCATCTCTGTCTCGCCTGTTCT |
Contig 1755 | UM04577 | Probable Urea Permease | F: GAAGGGGCTGGCTGGGCTGTAGT | F: TGCTTACGCGCGCCATCAATG |
| Â | Â | Â | R: GGCAAGACACCAACAGCGAGCG | R: GCGTCGAGTCCGGCAGCTTTG |
Contig 3380 | UM06012 | General Amino Acid Permease | F: GAAGCCAAGAACCCGCGCAAGT | F: TACCAACTACATCCCGATCCCCTTCTTC |
| Â | Â | Â | R: GTCGTGGGTTTCTCGTCTGGCTGTT | R: GTCGTGGGTTTCTCGTCTGGCTGT |
Contig 3396 | UM06045 | Probable Urease | F: ACTCCACCATGCCGGCTCCCTAG | F: CTCCATCCCCACAGTGCAGCCTATC |
| Â | Â | Â | R: GCGTCAATGGCAACTCGTCAGCTG | R: CCCCTTGACCCCCTCGATTCTCTT |
Contig 3235 | UM05889 | Ammonium Transporter UMP2 | F: GGCACGCCCACCGAACTCATCTAT | F: TCGACATCTTTGCTGCTCACGGTATTG |
| Â | Â | Â | R: TCGGTGGATGCAGTAGCGTGATGAA | R: TGGCGAACAGCAGGATGAGCGTAA |