Table 1 Primers for reverse transcription and amplification in the bovine SILV gene
Name | Positiona | Exon | Sequence (5' – 3') |
|---|---|---|---|
E1F3 | 69 | 1 | TGATGGGTGTTCTTCTGGCTG |
E3F1 | 280 | 3 | CTCTATTGCCTTGCACTTTCC |
E3R1 | 362 | 3 | CATTGATGATGGTGTTGTTGG |
E5R1 | 656 | 5 | TAATGGTGAAGGCTGAACTGG |
E5F1 | 575 | 5 | AACATGGAAGTGACTGTCTACC |
E7R1 | 1430 | 7 | AGCCATAGCGATACAGAACAC |
E7F1 | 1360 | 7 | GGATGACACTGCCACCTTAG |
E11R2 | 2046 | 11 | AGGGAAGACCAGAGAAAAGAC |
E6F2 | 952 | 6 | CACTACAGATAGGCATGTGAC |
E6R2 | 1030 | 6 | GCCCATGACTTCTGTAGTAGG |
E10F1 | 1773 | 10 | CTCCTCTGTTCGTGGGCATC |
E9F1 | 1643 | 9 | GTTTTGCACCAGGTACTGAAG |
RACE_E7N | 1441 | 7 | CAGGGTGAGGGAAAAGGAGCCATAG |
5UTRF1 | 8 | 1 | GTTGCTGGAAGGAAGAACAGG |
SPex23F1 | 88 | 1/4 | TGTAGGGACCACAGAAGGGAG |
E5R1 | 656 | 5 | TAATGGTGAAGGCTGAACTGG |
SPex56F1 | 481 | 4/7 | TGTCTGGAAGACCTGGGGCT |
E9R2 | 1587 | 9 | TGACACCCTGGCGATGAGATG |
E5F2 | 565 | 5 | GGGCACATATAACATGGAAGTG |
SPex6R1 | 1358 | 7/5 | GCAGGGGACTCAGGGAGCCAG |
SPex6AF1 | 798 | 6/7 | CCTACACCTGGGACTTTGGCT |
SPex6BF1 | 1167 | 6/7 | CAACTGCAAAAGCTACAGGCT |
E7F2 | 1409 | 7 | TGTGTTCTGTATCGCTATGGCTC |
SPex9R1 | 1767 | 9/10 | CTGAGGCCTGCTTCTTGCCCTG |