Tripartite chimeric pseudogene from the genome of rice blast fungus Magnaporthe grisea suggests double template jumps during long interspersed nuclear element (LINE) reverse transcription

Table 1 Expression of WEIRD and MGL relatively to housekeeping genes Ilv5 and Ef1

	Housekeeping gene	Mycelium	Spores	0 hours infection	8 hours infection	24 hours infection
WEIRD ^a	Ef1 alpha	1.6 ± 0.5	1.7 ± 0.8	-	1.0 ± 0.4	2.5 ± 0.8
	Ilv5	35. 2 ± 7.8	20.6 ± 5.2	-	35.0 ± 6.5	61.2 ± 12.2
MGL ^a	Ef1 alpha	2.8 ± 0.9	8.9 ± 2.0	-	1.3 ± 0.7	2.4 ± 0.4
	Ilv5	60.2 ± 14.5	108.2 ± 14.3	-	44.0 ± 6.6	58.2 ± 10.9

^aRelative transcription, % of the corresponding housekeeping gene transcript level. All quantitative RT-PCR experiments were performed in quadruplicate. Relative transcription was calculated according to the formula: 100% × 2^ΔC(t), where $2^{- Δ Ct} = 2^{- ({Ct}_{s a m p l e} - {Ct}_{r e f})}$ .

ISSN: 1471-2164