Tripartite chimeric pseudogene from the genome of rice blast fungus Magnaporthe grisea suggests double template jumps during long interspersed nuclear element (LINE) reverse transcription

Table 2 MINE-A, -B and -C expression relatively to housekeeping genes Ilv5 and Ef1

Tissue	MINE-A		MINE-B		MINE-C
	R.t.^a (Ef1alpha)	R.t.^a (Ilv5)	R.t.^a (Ef1alpha)	R.t.^a (Ilv5)	R.t.^a (Ef1alpha)	R.t.^a (Ilv5)
mycelium	4.4 ± 1.6	70.1 ± 14.5	4.4 ± 0.2	71.2 ± 5.1	1.4 ± 0.1	24.1 ± 8.5
spore	2.1 ± 0.4	20.0 ± 0.7	4.7 ± 0.5	44.6 ± 1.7	2.8 ± 0.3	26.4 ± 1.0
infection 24 hours	10.4 ± 1.0	154.3 ± 17.8	4.3 ± 1.24	64.4 ± 10.2	3.1 ± 0.6	42.7 ± 7.4

^aRelative transcription, % of the corresponding housekeeping gene transcript level. To specifically amplify each individual chimeric element, a combination of primers q1+q2 was used (See Figure 3). Primer q1 is specifically designed to the WEIRD-MGL junction site of the desired MINE element. All quantitative RT-PCR experiments were performed in quadruplicate. Relative transcription values were calculated as described on the legend to the table 1.

ISSN: 1471-2164