Table 7 Primer sequences used for PCRs
From: Gene profile analysis of osteoblast genes differentially regulated by histone deacetylase inhibitors
Gene | Strand | Primer Sequences | Annealing Temp (°C) | Product Length (bp) |
|---|---|---|---|---|
Akap12 | F | 5' GCCAGTGAAGAACATGAGCAG | 56 | 160 |
| Â | R | 5' CTATGCAATCTGCTTTGTCTTGG | Â | Â |
Ap4s1 | F | 5' GTACTTCAGCCGAGTGAGTG | 56 | 202 |
| Â | R | 5' CTCTTCCTTGGCCTTCACAG | Â | Â |
Amphiregulin | F | 5' GACTCACAGCGAGGATGACAAG | 56 | 249 |
| Â | R | 5' GCTTGGCAATGATTCAAC | Â | Â |
Fibroblast growth factor 7 | F | 5' CAGTTTGGAAAGAGCGACGAC | 56 | 170 |
| Â | R | 5' GGCAGGATCCGTGTCAGTATC | Â | Â |
Frizzled-1 | F | 5' CAAGGTTTACGGGCTCATGTAC | 56 | 180 |
| Â | R | 5' GTAACAGCCGGACAGGAAAATG | Â | Â |
Frizzled-4 | F | 5' CTGCAGCATGCCTAATGAGAG | 56 | 185 |
| Â | R | 5' CGTCTGCCTAGATGCAATCA | Â | Â |
Interleukin 1 receptor-like 1 | F | 5' CCAGCCAGAGTGGAAGACTC | 56.9 | 189 |
| Â | R | 5' CAGGTCAATTGTTGGACACG | Â | Â |
Psmb10 | F | 5' CTTTACTGCCCTTGGCTCTG | 56.9 | 168 |
| Â | R | 5' GTGATCACACAGGCATCCAC | Â | Â |
Qpct | F | 5' GAGGAGGCACTGAAGGAGTG | 53 | 162 |
| Â | R | 5' GAAGATCGGACTGGATGCTC | Â | Â |
Sdh1 | F | 5' CTGCCGATTCTACAAGCACA | 56.9 | 183 |
| Â | R | 5' AGCAAAGTGACCATCCCAAC | Â | Â |
Slc9a3r1 | F | 5' CAAAGTGTCCCCTCACCAGT | 56.9 | 209 |
| Â | R | 5' AATGAACCCAAGATGCCAAG | Â | Â |