Figure 5

Subcellular localization of human and mouse NEAT1 and NEAT2/MALAT-1. (A) Tig1 cells: Human NEAT1 RNA (green) distributes in discrete foci through the nucleus and is not detected in the cytoplasm. (B) Tig1 cells: NEAT1 RNA (red) is transcribed from 2 loci early in G1 (left) and occasionally shows a highly polarized pattern (right). (C) Tig1 cells: NEAT2/MALAT-1 RNA (red) is not detected in the cytoplasm at interphase and concentrates in domains in the nucleus with weaker signal apparent throughout the nucleoplasm. (D) NIH-3T3s: Mouse Neat2/Malat-1 RNA (green) localizes as bright nucleoplasmic signal in some cells and concentrates in domains in others. (E) NEAT1 RNA (red) does not localize to its parent chromosome 11 (green). (F) NEAT2/MALAT-1 RNA (red) rapidly disperses from its site of transcription (Chromosome 11 (green)) and distributes throughout the nucleus. (G) Tig1 cells: NEAT1 RNA (green) and NEAT2/MALAT-1 RNA are associated in the interphase nucleus. (H) MEFs: Mouse Neat1 RNA (red) is exclusively nuclear, and is found in fewer, larger foci than in human cells, yet shows the same peripheral association with Neat2/Malat-1 RNA (green), and neither RNA associates significantly with DAPI dense heterochromatin (blue).