Figure 6

Human and mouse NEAT1 and NEAT2/MALAT-1 are intimately associated with SC35 domains. (A) Tig1 cells: NEAT2/MALAT-1 RNA (red) is found in every SC35 domain (green); (B) NIH-3T3s: Neat2/Malat-1 RNA (green) concentrates in domains in about 50% of the population, when it does, it overlaps with SRM-300 speckles (red); (C,D,E) NEAT2/MALAT-1 RNA (green (C)) and a probe to poly(A) (red (D)) overlap (yellow (E)) in domains in the nucleus; (F) Neat1 RNA (red) preferentially localizes to the periphery of SRM-300 domains (green) in mouse cells; (G) NEAT1 RNA (red) preferentially associates at the periphery of SC35 domains (green) in human cells; (H) Two transcription sites of NEAT2/MALAT-1 RNA (red) localize with SC35 domains (green) in early G1 daughter cells; (I) Poly(A) domains (red) are apparently formed prior6=- to the spread of NEAT2/MALAT-1 RNA (green) in early G1.