Figure 2

The HyPRP families of potato and Arabidopsis . Left: an unrooted consensus phylogenetic tree (cladogram) of nucleotide sequences encoding the conserved C-terminal domains of HyPRPs from potato and Arabidopsis thaliana, constructed by the ML method (see Materials and Methods). Bootstrap values above 50 % (from 500 replicates) are shown above the individual branches, numbers below branches denote bootstrap values of a NJ tree from the same input data. The ML and NJ trees agreed in all clades with bootstrap support over 50 % with exception of (i) swapping St1 and At1g62500 and (ii) swapping At4g15160 and At4g15160, in both cases with NJ bootstrap values below 76 %. Arabidopsis loci are denoted by standard AGI locus identifiers. Gene names are color-coded and typographically marked according to the composition of the N-terminal domains of the encoded proteins as in Figure 1; in addition, proline- and glycine-rich N-terminal domains (not fitting into the categories of proline- or glycine-rich, but containing more than 10 % of each of these amino acids) are shown in blue. Arabidopsis genes with reliable potato orthologues are denoted by black filled squares, chromosomal clusters and tandem duplications are marked by chromosome cluster numbers to the right of the tree (see Additional file 2). Right: expression profiles of selected Arabidopsis HyPRP genes according to the publicly available expression data: top – orthologues of potato genes (with potato expression patterns from Figure 1 for comparison; apical stems shown in the "shoot apex" position), bottom – genes from the four Arabidopsis chromosomal clusters. Order of genes within each cluster corresponds to that in the tree.