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Table 6 Real-time PCR validation of microarray positive results

From: Differential gene expression profile in pig adipose tissue treated with/without clenbuterol

Gene* (Clone No. on Microarray)

Hog 3/hog 4

Sow 3/sow 4

 

Change (test/control)

P value

Change (test/control)

P value

ApoD (rpfat_18926)

12.28

0.013

7.49

0.002

PRKAR1A(rpfat_17661)

6.65

0.014

8.18

0.046

COL1A1 (rpfat_8523)

20.08

0.00008

13.47

0.00001

COL1A2 (rpfat_16033)

2.87

0.0010

3.66

0.0078

COL1A2 (rpfat_17393)

3.00

0.0002

2.29

0.0002

COL3A1(rpfat_18309)

Not detected

Not detected

Not detected

Not detected

COL3A1 (rpfat_19990)

Not detected

Not detected

Not detected

Not detected

  1. * The full names of each gene are listed in Tables 2-5.
  2. Five of the seven genes were confirmed by real-time PCR.