Table 14 Primers used for amplification of gene-specific DNA probes for slot-blot RNA hybridization.
Primer | Name | Amplified product | DNA sequence 5'→3' direction |
|---|---|---|---|
sll1336 | sll1336 | 1686 bps | ATGTCGTACTGAGTCGCTTC TGGAGTGCAACATGCTGGAC |
sll0902 | sll0902 | 627 bps | TCCTTCACCGCGGCCATGTA CGGCAGACAGTGGAGCACAA |
slr1476 | slr1476 | 986 bps | GGTGGCCAGTTGGACTCGAA ATTCCTGAACAGTGCCTAGC |
sll0573 | sll0573 | 491 bps | AACGGAAGGCATGATCGGTT AACAGTGAGCGTAGTTGGTG |
slr0782 | slr0782 | 1325 bps | CCATCCTCGTCCTGTGATTG CCAGTACGAATTGCACCATC |
sll1077 | speB2 | 1054 bps | CAGCAGGAGGTTGACCAAGG CAGCATGGATATAGGCCGGT |
slr1022 | argD | 1224 bps | GTTGTTGAATCCGTCGAAGC TTCTGCTTCCGTCACCACTA |
slr0370 | gabD | 895 bps | GCCGAGGAATACTTAGCCGA GGTTAGTTGTCCATGCACTG |
sll1683 | sll1683 | 858 bps | ACCTCTTCCAAGCTGATCTG AGGCAGTGACATCGACGGTA |
slr0662 | slr0662 | 739 bps | GTTGGACCATTGACGACAGC CTGTCCAACATATCAGCTCG |
slr1312 | slr1312 | 853 bps | GCCTCCTGGAGCATTGAAGA CCAGCTTGACCAATTCCACA |
slr1469 | rnpB | 599 bps | GCGGCCTATGGCTCTAATCA TTGACAGCATGCCACTGGAC |