Figure 7

RNA gel blot hybridization analysis of four selected genes. Total RNA (10 μg) from cotyledons of the reference tissue (S1) and the subsequent stages (S2–S7) was prepared in 6.5% formaldehyde and 50% formamide, electrophoresed on a 1.2% agarose, 1.1% formaldehyde gel and blotted onto a nitrocellulose membrane [61]. The blots were hybridized to radioactive probes obtained from the soybean public EST collection that contained the specific oligo sequence to be validated. These clones were hand picked, grown, purified, confirmed by sequence analysis and radioactively labeled. The relative values obtained by microarray data analysis are reported at the bottom of each lane.