Table 4 Characteristics of genes amplified for SNP detection
Genes | Chromosomal Location | Accession no. | Function | Forward primer | Reverse primer | Product length | No of SNPs |
|---|---|---|---|---|---|---|---|
4G21 | X | AY648704 | Cytochrome P450 | GGCGATAGCAAACGTAAAGC | CGCGGTAAACGGAATATAGC | 303 | 2 |
9K1 | X | AY987362 | Cytochrome P450 | GTACGAGCTGGCCGTTAATC | CCTTTCTGTAGCTGCACCTTG | 243 | 9 |
4J12 | 3R | AY648706 | Cytochrome P450 | CCAACAAATCAGTTCATCAGC | TTGTAAAAGTGCTTAAAATG | 270 | 9 |
6P9 | 2R: 9A-12C | AY729661 | Cytochrome P450 | GCGCCTTAGACAAGAGATCA | AAGGGATGTCGCTTCTTCTC | 350 | 8 |
6P4 | 2R: 9A-12C | AY987359 | Cytochrome P450 | GTACGAGACTGGCAAAGAAT | AAGGAAGACGTATGGATGG | 430 | 13 |
6P5 | 2R: 9A-12C | AY987360 | Cytochrome P450 | CTGGCTTTGAAACTTCCTC | AGATACACGTAGGGATGTCG | 550 | 3 |
6Z1 | 2L: 25A-27D | Â | Cytochrome P450 | ACGATCCGTTCCGGGTAG | GCTAGCGCAGGATACATTCG | 550 | 12 |
6Z3 | 2L: 25A-27D | Â | Cytochrome P450 | GACGATCCGTTCCTGAAGAC | ATCGGTAAGCCCGGATATTT | 550 | 20 |
9J12 | 3L | AY729663 | Cytochrome P450 | TACCGGTGTGCAGCTTGA | CTTTGGCGCGAAGGTAAA | 194 | 5 |
9J14 | 3L | AY729665 | Cytochrome P450 | CGGACAACGTATGATCGATTT | TTTGGCTTGCATTAAAAGGTG | 214 | 5 |
BU01 | X:2B | BU039001 | type II transforming growth factor-beta receptor | GTGTGTTTGCTTGGGTGTTG | GGCATCGGTAATCAGGATGT | 525 | 1 |
BU08 | 2R:7C-10B | BU038908 | rhodopsin | CATTTGTGGAACCCCATTTC | GGTCATTGGTTTACCCGAGA | 500 | 7 |
Ache | 2R:9C-12C | DQ534435 | Acetylcholinesterase | GGGTACGGGACAACATTCAC | CGTTAACGTACGGGTCGAGT | 1050 | 15 |
BU10 | 2L:28A | BU039010 | Cyt-c-p-P1 | AAGCACAGTTAAACCTTTCG | ACCTAGCCCAATCTCTGTCT | 650 | 10 |
BU11 | 3L:43B | BU038911 | protein transporter | ATCTGCTTGCGCTAGATCGT | ATCGCCAAATTTCATCTTCG | Â | 2 |
BU12 | 2R:7B | BU038912 | Alpha tubilin | AAGCTCGAGTTCGCCATCTA | CTCCAATCCTTTCCGACGTA | 800 | 5 |
BU13 | 2R:15C | BU038913 | signal sequence receptor | ACCCTGAGAAATCGTAACAA | CCGATAGTTGAGAGCAATGT | 630 | 10 |
BU19 | 2R:12B | BU038919 | Chitinase | CTGTTGCTGCTGCTACATAC | CCGGTCACGTACAAATAGTC | 670 | 10 |
BU021 | 3L:38C-40B | BU039021 | Tubilin beta-3 chain | GAGTTGGTTGATGCCGTGTT | CGTCCGGAAACAAATATCGT | 400 | 8 |
BU21 | X:3A | BU038921 | Phosphoribosylaminoimida-zole carboxylase | TTTCAAGGTGAACGGTGTGA | CCATCAAGATGACGACCAGA | 475 | 11 |
BU25 | 2R 12B | BU038925 | ferritin heavy chain-like protein | GCGTAAAGCTGTCGTCCTTC | ATTCCCCCGTCAGGTAGTCT | 1200 | 2 |
BU29 | 2L:27B | BU038929 | sensory appendage protein | CACCAAGTACGATGGTGTCG | AGGCACTTGGTTTTGCAGTT | 410 | 6 |
BU34 | X:1C | BU038934 | NADH dehydrogenase | GGCAGGTAGCAGCAGTTTTC | CAGTACCAACCGCAACACAC | 400 | 6 |
BU35 | 2R 12B | BU038935 | CG6846 gene product | TTCAGCAAACACGTTTCGTC | ACTTGCCCTTGTCCTTGTTG | 400 | 9 |
BU40 | 2R:14B | BU038940 | Glutathion peroxydase | AGGCAAAATCAATTTTTGAA | CGTAACAATTTCTCGACCAT | 1150 | 10 |
BU56 | 2R:7B | BU038956 | novel An. gambiae salivary protein | AATCTAGAAGCTGCGCCAGA | AATTCTAGGACGGCGATTCC | Â | 13 |
BU58 | 2R:12D | BU038958 | translation initiation factor | ACTTCCACGCCCAGTGTATC | CGTGCAGAGTTCGAAAACAA | 650 | 5 |
BU62 | 2L:23A | BU038962 | cAMP responsive element binding protein | CAATCGGAGCGTAAGGAAAG | CGTTCTCCCGCAAAAACTAA | 475 | 13 |
BU66 | 3R:30A | BU038966 | Lysozyme | TAGCTCATAGTGGCGGTTAT | ACTACAACATGTCGTGCAAA | 650 | 22 |
BU70 | 2R:7C | BU038970 | Ubiquitin fusion 80 | GTGGACTCCGTACCTGGTCA | CTGTAGAATTACAGGAGGGCGTA | Â | 5 |
BU71 | 3L:39A | BU038971 | structural protein of peritrophic membrane | GGGAAGTCGGTGTAGGGAAT | ACGTTTGGGTCAGGTAGTCG | 750 | 11 |
BU72 | 2R:12B | BU038972 | RHO small monomeric GTPase | GATGAAGCTGCCAAAGATCC | TGCCTCGTCGAAAACTTCTT | 900 | 11 |
BU73 | 2R:7A | BU038873 | actin binding | AGTAAGAAACGAACGCAAAG | CGGAAAAGTTGGAATGTAAC | 430 | 3 |
BU76 | 2R:10B | BU038976 | translation initiation factor | TGCCTACGAACGACGTAATG | GGCTCGTAGCTGGTCACTTC | 500 | 16 |
BU77 | 2R:10C | BU038877 | ubiquitin conjugating enzyme | CAACACACTAGCCAGCAAGG | TTTGGTTCGGCCAACATACT | 408 | 23 |
BU82 | 2R:14D | BU038882 | Unknown | AGGGCGGTACAACAAAATCT | GCATCGGAGCGTTTCCTA | 400 | 8 |
BU85 | 2R:12E | BU038885 | phosphoglycerate mutase | AAAAAGAATGGCCGGAAAGT | CTCATCGCCCAGAATTTCAT | 800 | 14 |
BU88 | 2R:11B | BU038988 | translation initiation factor | GTGGCCTCCCACTTTGTTAG | TACCGGATACGGTTGACGAT | 800 | 29 |
BU90 | 3R:35B | BU038990 | gustatory receptor | GGGACATCATCATCATCGAC | TTTCGCTTCTCGCGTTAAAT | 300 | 3 |
BU92 | 3L:39A | BU038892 | Microtubule binding | CATGCGACCGAAGAGAAGTT | ATCCTGATTCTGGCTCATGG | 550 | 18 |
BU93 | 2R:7C-10C | BU038893 | prefoldin subunit 2 | CACCGGAAACTCGGCTATTA | TATCGGTTCCATCCGAAAAG | 550 | 10 |
BU98 | 3L:46B | BU038898 | CG7630 gene product | TGCGTCACCCGTTACAAATA | ACGTGTACGCTTTCCACCTC | 550 | 15 |
BU883 | 3R:32B | BU038883 | peritrophin | TTCGTGACACAGTTATACGC | GCACACTTCAGACTTCCTGT | 650 | 22 |
BU897 | 3R:36C | BU038897 | NADH dehydrogenase (ubiquinone) | GGGAATTCCGTGATTTTT | GGCAGAAATATCCATAATCG | 700 | 15 |
BU901 | 2L:20C | BU038901 | CG18397 gene product | AAAGACACTCCCGCATTACG | CTCGTGTCTGTTTGGCTTGA | 480 | 7 |
BU973 | 3R:36F | BU038973 | polyA-binding protein II | AGTAAGAAACGAACGCAAAG | CGGAAAAGTTGGAATGTAAC | 630 | 3 |
BU974 | 3L:40A | BU038974 | serine-type peptidase | ACTGGCGGAGAACGTACAAC | TGCTGCACATTAATCAAAGGTT | Â | 4 |
BU982 | 2R:12B | BU038982 | ferritin 2 light chain homologue | CTAGTTTCCTGTCGCGTTCC | CATCGTCTCCTCCATTACCG | 400 | 3 |
BU996 | 2R:8D | BU038996 | vacuolar hydrogen-transporting ATPase | GTTCGCCTACATGTGCTTCA | ACAAAGGGTGTGCAAAAAGG | 800 | 21 |
Kdr | 3R: 36A-37E | DQ534436 | Sodium channel gene | TGCAAAATAGAGTCATTGGTGAA | ATCATCTTCATCTTTGC | 1342 | 9 |