An annotated catalogue of salivary gland transcripts in the adult female mosquito, Ædes ægypti*
© Ribeiro et al; licensee BioMed Central Ltd. 2007
Received: 05 August 2006
Accepted: 04 January 2007
Published: 04 January 2007
Saliva of blood-sucking arthropods contains a cocktail of antihemostatic agents and immunomodulators that help blood feeding. Mosquitoes additionally feed on sugar meals and have specialized regions of their glands containing glycosidases and antimicrobials that might help control bacterial growth in the ingested meals. To expand our knowledge on the salivary cocktail of Ædes ægypti, a vector of dengue and yellow fevers, we analyzed a set of 4,232 expressed sequence tags from cDNA libraries of adult female mosquitoes.
A nonredundant catalogue of 614 transcripts (573 of which are novel) is described, including 136 coding for proteins of a putative secretory nature. Additionally, a two-dimensional gel electrophoresis of salivary gland (SG) homogenates followed by tryptic digestion of selected protein bands and MS/MS analysis revealed the expression of 24 proteins. Analysis of tissue-specific transcription of a subset of these genes revealed at least 31 genes whose expression is specific or enriched in female SG, whereas 24 additional genes were expressed in female SG and in males but not in other female tissues. Most of the 55 proteins coded by these SG transcripts have no known function and represent high-priority candidates for expression and functional analysis as antihemostatic or antimicrobial agents. An unexpected finding is the occurrence of four protein families specific to SG that were probably a product of horizontal transfer from prokaryotic organisms to mosquitoes.
Overall, this paper contributes to the novel identification of 573 new transcripts, or near 3% of the Æ. ægypti proteome assuming a 20,000-protein set, and to the best-described sialome of any blood-feeding insect.
Ædes ægypti is a highly anthropophagic and cosmopolitan mosquito vector of epidemic dengue and yellow fever. To achieve fast blood feeding, adult female mosquitoes inject a complex salivary mixture into their hosts while probing for blood. Mosquito saliva, like that of other blood-feeding animals, has antihemostatic and antiinflammatory activities that counteract host responses that would otherwise restrict blood flow or call the attention of the host to the feeding site [1, 2]. A preliminary transcriptome of adult female salivary glands (SG) has been previously reported  where 32 full-length transcripts have been described based on an analysis of 456 expressed sequence tags (EST). Of these putative proteins, ten have been verified by Edman degradation of Coomassie blue-stained bands from sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS-PAGE) of SG homogenates. Most salivary proteins found have no known function.
The genome of Æ. ægypti has been recently made available, facilitating further gene discovery. In this paper, we present the analysis of an additional set of 3,776 SG cDNA sequences (total of 4,232 compared with previous set of 456 clones). We describe 573 new transcripts, 136 of which code for proteins of a putative secretory nature, most of which have no known function. We expect this work will contribute to the understanding of the evolution of blood feeding in arthropods and to the discovery of novel pharmacologic agents.
Results and discussion
General description of the salivary transcriptome database
A total of 4,232 clones was included in the EST salivary database, including 456 previously described . The average length of the sequences was 752 bp, with 1419 sequences containing a polyA signature (20 contiguous A's). These clones assembled into 1,273 clusters (containing 2–261 sequences per cluster) and singletons (956 sequences). In this paper, we will use the word 'contig' to refer to clusters of one or more sequences. Mitochondrial sequences, identified by their match to sequenced Aedes albopictus and anopheline mitochondrial genomes, accounted for 73 EST from 13 clusters (thus far, the mitochondrial genome of Æ. ægypti is unknown). Of interest, several of these mitochondrial sequences mapped to scaffolds named supercont1.593 and also to supercont1.600, supercont1.929, and supercont1.363. Upon close inspections, these genomic scaffolds contain large segments of high similarity to Ae. albopictus mitochondrial genome. Accordingly, these contigs may be assigned to the mitochondrial genome in the final genome assembly, or may represent translocation of Ae. aegypti mitochondrial genes to the nuclear genome. To attempt a functional classification of these unique sequences, we compared them with proteome databases by blastx and with protein motifs by rpsblast (see Methods). Following manual annotation of these contigs, which included assignment of known or putative functions to the translation products, they were further divided into four categories: secreted (Sclass) with 352 contigs and 2,723 sequences; housekeeping (Hclass) with 739 contigs and 1,264 sequences; transposable element (Tclass) with 5 contigs and 9 sequences; and a last category composed of contigs coding for proteins of unknown function (Uclass) with 177 contigs and 234 sequences. The unknown class may contain truncated transcripts mainly mapping to 3' untranslated regions of genes. Although the Sclass corresponds to only 27% of the contigs, it consists of 64% of all EST, reflecting the relatively low complexity and abundance of the secretory material of the SG, as indicated before .
Transcribed transposable elements
Nine transcripts in our database possibly derive from transposable elements. Their translation products are similar to those of the sea urchin Strongylocentrotus purpuratus  and to Tc1-like transposase. These transcripts may indicate active ongoing transposition activity in Æ. ægypti or, more likely, they may represent regulatory elements suppressing transposition of relatively recent genome invasions.
Housekeeping gene products
Functional classification of housekeeping transcripts.
Protein export machinery
Carriers, Transporters, Channels
Amino acid metabolism
Unspecified membrane proteins
Updated catalogue of salivary proteins
We used the transcriptome set (Additional File 1)  and the Artemis tool to identify novel proteins coded in the Æ. ægypti genome. We have also assembled our transcripts with a set of ~220,000 EST of Æ. ægypti available in public databases to obtain eventual full-length information of translation products. This assembled EST dataset, similarly organized as that for AnoXcel , is available online . Using these tools, we identified 614 protein sequences, mostly full length, deriving from the salivary transcriptome of adult female Æ. ægypti mosquitoes. Of these 614 protein sequences, 573 were identified and contributed to GenBank from this work, and the remaining were previously known. Of these 614 proteins, we identified a set of 136 putative secreted proteins expressed in the SG of Æ. ægypti, 97 of which are novel (Additional File 2) .
Classification of 71 selected genes encoding putative secretory products as determined by RT-PCR expression analysis on female salivary glands, whole male mosquitoes, and carcass of female mosquitoes
Protein name or type
Secreted carrier-like proteins including D7 family
Secreted protease inhibitors
Similar to FXa-directed anticlotting
Other protease inhibitors
Second apyrase or 5' nucleotidase
Salivary purine nucleosidase
Putative adenosine deaminase
Salivary chymotrypsin-like enzyme
Putative serine protease
Putative salivary C-type lectin
Putative C-type lectin
C type lectin
Angiopoietin-like protein splice variant
Putative salivary peptide with HHH domain
Gram-negative binding protein
Peptidoglycan recognition protein
Unknown function, secreted, and ubiquitous
Antigen 5 protein family
Antigen 5 member
Putative secreted protein
Antigen 5 family member
Other secreted proteins found in non-bloodsucking insects
Cysteine-rich venom-like protein
Unknown function, found in hematophagous Diptera salivary transcriptomes
Putative 56.5 kDa secreted protein
41-kDa mosquito family
Putative 41-kDa salivary secreted protein
30-kDa salivary gland allergen variant 2
Putative 30-kDa allergen-like protein
Putative salivary protein
Other salivary proteins found in hematophagous Diptera
Putative secreted protein
Similar to An. gambiæ gSG8 protein
Putative 8.9-kDa secreted protein
Putative 23.4-kDa salivary protein
Putative secreted peptide
Putative secreted peptide
Possible secreted protein
Putative secreted salivary peptide
Aedes genus specific
62kDa family – Single exon
Putative secreted protein
Putative secreted protein
34-kDa gene family
Salivary peptide similar to 34-kDa protein
Putative salivary peptide
Putative 34-kDa secreted protein family
Putative 34-kDa secreted protein
Putative 30.5-kDa secreted protein
Putative 30-kDa secreted protein
Salivary peptide with WWW domain
Putative secreted peptide
Other Aedes-specific polypeptides
Putative salivary secreted peptide
Putative salivary peptide with basic tail
Putative 18.6-kDa secreted protein
Putative 18.6-kDa secreted protein – variant
Putative 7.8-kDa secreted protein
Putative basic peptide 4.2K-1
Putative salivary secreted peptide
Putative 8.7-kDa secreted protein
Peptide similar to insulin-like growth factor
Secreted salivary proteins
Proteins with some function confirmed or presumed from structure
Secreted ligand carrier-like proteins including D7 family
D7 salivary proteins
The first D7-coding gene was reported 15 years ago, for the mosquito Æ. ægypti . It was later found in virtually all mosquito sialotranscriptomes where short (~15 kDa) and long (~30 kDa) forms were recognized . In An. gambiæ, this gene family, except for one poorly transcribed gene, appears to be selectively expressed in the female SG [17, 18], indicating a role in blood feeding. This protein family is distantly related to the odorant-binding protein (OBP) family, which specializes in binding small ligands . Recently, some mosquito D7 proteins were shown to bind and inhibit the action of biogenic amines such as serotonin, histamine, and norepinephrine, a function that might help blood feeding . Additionally, one short D7 protein from Anopheles stephensi, named hamadarin, was shown to prevent kallikrein activation by FactorXIIa . Long D7 forms also exist in sand flies  and Culicoides , indicating that this gene family was recruited very early in the evolution of hematophagous Nematocera.
Phosphatidylethanolamine binding proteins
We report three members (one possibly truncated) of this very ubiquitous protein family never found before in sialotranscriptomes of blood-sucking arthropods [26, 27], two of which have clear signal peptide indicative of secretion. This protein family is known to bind lipids and was also shown to have serine protease inhibitory capacity. Their role in saliva is unknown. No enrichment was found for these transcripts on the SG libraries when compared with other libraries.
Other small molecule binding proteins
An OBP and a lipocalin with a juvenile hormone binding motif were found in the sialotranscriptome of Æ. ægypti, both containing distinct peptide signal indicative of secretion. The D7 proteins belong to the OBP superfamily. Lipocalins are abundantly expressed in tick and triatomine sialomes, where they act as nucleotide (nt)-and biogenic amine-binding proteins in addition to other functions. Their function in Ædes is unknown. No enrichment was found for these transcripts on the SG libraries when compared with other libraries.
Secreted protease inhibitors
Two serpins have been described before in Æ. ægypti, one of which has been characterized as an inhibitor of FactorXa of the clotting cascade [28, 29]. We present one allele of the FXa-directed anticoagulant precursor having only 89% identity to the reported protein which originated from the Rockefeller strain of Æ. ægypti, and two alleles of a novel salivary serpin mapping to supercontig1.65. The three genes coding for these serpins are not located near each other in the Æ. ægypti genome. All three salivary Æ. ægypti serpins have corresponding homologues found in Ae. albopictus sialotranscriptome. The novel serpin has abundant tryptic fragment matches recovered by proteomics (band marked Serp2, Figure 4), indicating its expression in the SG, as did gi|18568304 (marked Serp1, Figure 4). Transcripts for all serpins are significantly overrepresented in the SG library when compared with the remaining libraries, in accordance to the RT-PCR experiments shown in Table 2, which indicates that two of the three serpins are female specific and that one may be found also in males but not in carcasses of females not containing SG (Figure 5).
Other protease inhibitors
A Kazal domain-containing peptide, similar to one described in Ae. albopictus and to several other proteins described as thrombin inhibitors, was found in the Æ. ægypti sialotranscriptome. A cystatin was also found, but this protein is reported as truncated; we were not successful in searching the genome for the missing exon(s). This could be due to the large intron size observed in Ae. aegypti. Accordingly, no indication of secretion is possible, but it is described in this section due to the importance of this family in inhibiting proteases associated with inflammation. Both transcripts are ubiquitously found in mosquito tissues by RT-PCR and may play a housekeeping role.
The gene coding for this endothelium-dependent peptide vasodilator [32, 33] has been reported earlier and shown to be transcribed specifically in female SG . Although two forms of the peptide have been described earlier differing in the aminoterminal (aspartate or asparagine), only one gene is found coding for this peptide sequence, for which 60 EST were found in the salivary cDNA library. The Asn version may have been an artifact from the modification of the original peptide, which was stored in acidic solution.
A novel ribonuclease of the T2 family was also characterized. This enzyme has not been previously characterized in sialotranscriptomes. It has a typical signal peptide indicative of secretion and may function in the degradation of extracellular RNA .
Nine secreted serine proteases varying in predicted mature molecular weight between 28 and 43 kDa were found in the Æ. ægypti sialotranscriptome, seven of which are being reported for the first time (Additional File 2). Two of these serine proteases (AEA-876and AEA-562) contain a CUB domain, indicating specialized substrate recognition. Both are found in supercontig1.217 within 63 kb of each other. Some of these enzymes (such as gi|18568334) are possibly related to immunity and are similar to other enzymes annotated as prophenoloxidase (PPO)activators, but they could have been co-opted to function in hydrolyzing specific host proteins. A smaller number of this type of enzymes found in An. gambiæ sialotranscriptomes was selectively expressed in the SG of adult females, indicating they may play a role in blood feeding, perhaps by activating antiinflammatory pathways (such as protein C) or deactivating inflammation. One such Ædes enzyme is AE-226, similar to proteins annotated as chymotrypsin, which is overexpressed in Ædes sialotranscriptomes as opposed to the remaining transcriptomes. Three of these serine proteases were significantly underrepresented in the nonsalivary-gland libraries. Four serine proteases tested by RT-PCR agreed with the library frequency results: the transcripts were found in female glands and adult males but not in female carcasses deprived of SG.
Previously reported amylase[51, 52] and alpha-glycosidase/maltase[53, 54] are abundantly overrepresented in the salivary EST collection. These genes were shown to be expressed in the proximal regions of the female glands, the region associated with sugar feeding.
An alkaline phosphatase and a carboxylesterase, both containing signal peptides indicative of secretion, are described. Both enzymatic activities in adult female SG have been previously described in Ædes , and the esterase activity shown in saliva, but their function in blood feeding can only be speculated upon.
The SG of mosquitoes produce various antimicrobial polypeptides and other immunity-related products such as bacterial surface-recognizing proteins and lectins that may be important in opsonization and initiation of activation of the PPO enzyme leading to pathogen melanization. The purpose of these products may be to control microbial growth in the sugar solutions stored in the crop or in the gut following a blood meal. Previously, lysozyme was found in both male and female SG of Æ. ægypti, and in the mosquito crop  and shown to be secreted by females following either a sugar or blood meal . Indeed, Æ. ægypti salivary lysozyme is significantly overrepresented in the SG libraries, and the RT-PCR experiment supports salivary expression in both male and female SG (Table 2 and Figure 5). Anopheline mosquitoes also display a similar pattern of lysozyme expression in the proximal gland region . It is possible that most immunity-related gene products follow the same pattern of expression shown by lysozyme. Some of the enzymes possibly associated with PPO activation have been listed above in the Enzyme section.
Five C-type lectins are described in Additional File 2, four of which are novel. Expression of AE-189 and gi|18568318 was confirmed by mass spectrometry following tryptic digestion of protein bands (labeled C-Lec1 and C-Lec2 in Figure 4). These two lectins are also expressed significantly more in sialotranscriptomes than in other Æ. ægypti cDNA libraries, indicating that they are possibly salivary-tissue specific. In accordance with this expectation, RT-PCR experiments indicate expression exclusively in female SG (Table 2 and Figure 5). The two genes coding for these salivary-specific lectins were found as an inverted tandem repeat in supercontig1.10, each with a single intron separating the signal peptide gene region from the remaining coding sequence. Two other C-type lectins tested by RT-PCR were ubiquitously expressed.
The C-type lectin family is expressed in most mosquito sialotranscriptomes described thus far. This protein family is implicated in immune recognition phenomena in general and in Plasmodium development in Anopheles in particular [62, 63]. Despite these not-yet-demonstrated roles of C-type lectins in salivary immunity, it is interesting that in snake venoms this protein family has been recruited to perform various unrelated functions such as anticlotting, toxin, and platelet aggregation inducer [64, 65]. Lectins may also play a role in the colectin pathway of complement activation . Hemagglutinins were described in anopheline SG more than 60 years ago . This activity may help concentration of red blood cells in the mosquito gut . The molecular nature of any anopheline hemagglutinin, however, is unknown. Differently from anophelines, and despite having salivary lectins, Ædes SG homogenates lack hemagglutinins, indicating that the salivary lectins do not recognize vertebrate red blood cells or that they are monomeric in their carbohydrate binding site. Overall, it appears that the two female SG-specific lectins may have a role in hemostasis rather than immunity.
Other proteins with sugar-binding domains
AET-12005 and AET-670 are similar to, but shorter than, N-acetylgalactosaminyltransferase and glucuronyltransferase, respectively, appearing to derive from novel genes that arose from gene duplications and partial deletions of ancestral genes coding for carbohydrate binding enzymes, the final products lacking the original carboxyterminal domain. AET-12005 has a partial Pfam Glycos_transf_2 motif that comprises a diverse family transferring sugar from UDP-glucose, UDP-N-acetyl-galactosamine, GDP-mannose, or CDP-abequose to a range of substrates including cellulose, dolichol phosphate, and teichoic acids. AET-670 has a weak match to the PFAM UDPGT motif and is similar to proteins in the nonredundant (NR) database annotated as UDP-glucosyl transferase. It is possible that these proteins have a destination in the endoplasmic reticulum or Golgi and do not have a secretory nature. Their function is unknown.
The proteins annotated as imaginal disk growth factor protein 4 and AEA-871BRE were expressed in the sialotranscriptome of Æ. ægypti. These proteins have a chitinase domain and are homologous to An. gambiæ bacteria responsive protein 1 and bacteria responsive protein 2, which were shown to be immune-responsive chitinase-like proteins that have lost chitin-binding activity .
This group of proteins has the PFAM fibrinogen C motifseen in invertebrate proteins displaying lectin activity toward N-acetylglucosamine residues and implicated in immune function . In An. gambiæ, the ficolin family was expanded in comparison to Drosophila melanogaster, where 53 members were seen in its genome as opposed to 20 in the fruit fly . Three proteins belonging to this family are shown in Additional File 2, two of which are novel. Evidence for salivary expression of gi|18568298 and AE-154 was found by mass spectrometry in tryptic digests of protein bands (labeled Ang1 and Ang2, respectively, in Figure 4). Of interest, the two genes for these proteins occur as a tandem repeat in supercontig1.15. EST for these two genes are also overrepresented in the sialotranscriptomes and are indicated to be female-salivary-gland specific by RT-PCR experiments, thus suggesting a blood-feeding rather than an immune role for these proteins.
Antimicrobial peptides (AMP)
The gene products for the AMP gambicin, lysozyme, and defensin A1 have been previously described in Æ. ægypti sialotranscriptomes and are listed in Additional File 2. Transcripts encoding gambicin and defensin A1 were detected by RT-PCR in all tissues examined, indicating ubiquitous expression (Table 2 and Figure 5); however, a significant overrepresentation of the corresponding EST in sialotranscriptomes should be pointed out. We additionally describe three novel peptides that may have an antimicrobial function. AET-590 has GY repeats that are also found in peptides of similar size known to have antimicrobial activity in nematodes . AET-462 and AET-11358 are candidate AMP containing a HHH motif seen in other histidine-rich AMP [82, 83]. AET-11358 appears to be SG specific, as a total of 88 EST was found in the combined SG transcriptome, although none were seen in other tissue transcriptomes. RT-PCR confirmed the presence of the transcript in female glands and male bodies but not in female carcasses without SG (Table 2 and Figure 5).
Other immune-related gene products
A peptide (named AEA-233) closely related to a previously described Æ. ægypti peptide named i23R potentially involved in Plasmodium susceptibility  was found in the sialotranscriptome. We also present an allele to AEA-233a, indicating the polymorphism of this gene. The Æ. albopictus sialotranscriptome revealed a homologue that is 63% identical, but no significant matches were found to any other animal or plant proteins in the NR database. This peptide may belong to a not-yet-characterized antimicrobial gene family specific to the Ædes genus. Expression of AEA-233a was ubiquitous by RT-PCR.
Two other gene products are described, both associated with pathogen surface-pattern recognition: the previously described Gram negative binding protein, which is significantly overrepresented in sialotranscriptomes and appears expressed both in female SG and in adult males (Table 2, Figure 5), and the novel AE-7210, which is similar to peptidoglycan recognition proteins and was ubiquitously expressed by RT-PCR experiments.
Mucins and peritrophins are proteins associated with lining of epithelia or inert extracellular structures, such as chitin. Mucins are highly glycosylated proteins containing Ser or Thr modified with N-acetylgalactosamine residues. Their expression in the SG may have a function of lining the chitin surfaces of the mouthparts, but they may also assist in antimicrobial functions.
We present 12 mucins in Additional File 2, 11 of which are novel, including one allele. These proteins have an average Ser+Thr equal to 13.8% of their total aa, as opposed to 0.9% observed as the average of all proteins found in Additional File 2. We additionally report on a polypeptide (AE-466, mucin-like peritrophin) containing three glycosylation sites and one chitin-binding domain, which may be involved in proximal lining of the cuticular duct. All other proteins have 11–69 glycosylation sites.
Putative secreted proteins without functional classification
Belonging to ubiquitous protein families
Antigen5 (AG5) family
AG5-related salivary products are members of a group of secreted proteins that belong to the CAP family (cysteine-rich secretory proteins; AG5 proteins of insects; pathogenesis-related protein 1 of plants) . Members of this protein family are found in the SG of many blood-sucking insects [3, 90, 91]. Most of these animal proteins have no known function; in the few instances to the contrary, they diverge from proteolytic activity in Conus , to smooth muscle-relaxing activity [93, 94] in snake venoms, to salivary neurotoxin in the venomous lizard Heloderma horridum . Three members of this gene family were previously described in the sialotranscriptome of Æ. ægypti. EST's for all three genes are overrepresented in the sialotranscriptome as compared with the combined transcriptomes, indicating they may be preferentially expressed in the SG. In accordance with these results, gi|18568284 was exclusively transcribed in female glands as indicated by RT-PCR, suggesting an antihemostatic function for the gene product, while the other two genes are transcribed in female glands and male bodies but not in female carcasses.
Differently to An. gambiæ, which has four salivary AG5 members, three of which cluster in chromosome arm 2 L , the salivary AG5 proteins of Ædes do not appear to cluster in the genome, mapping to different supercontigs.
Other secreted proteins of unknown function found in non-bloodsucking insects
Eight putative secreted proteins have similarities to proteins or protein domains found in non-bloodsucking insects. One of these proteins (AE-796)  is a truncated fragment where it is not possible to identify whether it has a signal peptide indicative of secretion, but it has a weak CDD domain PAN_AP_HGF, which is found in plasminogen/hepatocyte growth factor proteins, and various proteins found in Bilateria, such as leech antiplatelet proteins; however, the mRNA encoding this protein was found ubiquitously expressed by RT-PCR and may not have a unique salivary role in blood feeding. AE-389 has a TIL domain (t rypsin i nhibitor l ike) and is significantly overexpressed in sialotranscriptomes. RT-PCR indicates both male and female SG may be the target tissue of expression of this peptide (Figure 5 and Table 2). Peptides containing a TIL domain were also found in the An. stephensi  and An. gambiae adult male sialotranscriptomes[101–103]. The finding of this type of peptide being overexpressed in male An. gambiæ SG indicated a possible antimicrobial function rather than a function as a host serine protease inhibitor during blood feeding. Indeed, a tick TIL domain containing peptide named ixodidin was found to have an antimicrobial function in addition to inhibiting serine proteases . The remaining six polypeptides have similarities to Drosophila or other species, and their structure does not hint at any particular function.
Belonging to families only found in blood-sucking diptera
This protein family has been found to date only in salivary transcriptomes of adult mosquitoes, including adult male An. gambiæ. The SG specificity of this gene transcript in Æ. ægypti is supported by significant overrepresentation of EST on the sialotranscriptome and by RT-PCR (Figure 5 and Table 2). All family members have a signal peptide indicative of secretion and a predicted molecular weight near 56 kDa. BLAST comparisons also show weak similarity to bacterial proteins but to no other eukaryotes. Following 4 iterations of PSI-BLAST, only mosquito and bacterial proteins are retrieved , suggesting that this family of proteins may have originated as a lateral transfer from a bacterial genome to the ancestral mosquito genome. The single exon structure of the gene – unusual in eukaryotes, particularly for a protein of this size, but the rule in prokaryotes – supports this hypothesis. The An. gambiæ homologue also displays a single exon gene structure , as reported previously . The bacterial proteins retrieved by PSI-BLAST are mostly annotated as phage-associated proteins, suggesting the lateral transfer might have occurred via a phage-associated mechanism.
Two novel alleles coding for this protein family are described in Additional File 2. The gene coding for this protein has a 3 exon structure on supercontig1.116. The gene product shows similarities to proteins of comparable size found in Æ. albopictus and Culex pipiens and to a shorter protein in salivary transcriptomes of Culicoides sonorensis. A match to Æ. ægypti gi|61742035 probably represents a misannotated protein with a DNA frame-shift error. Three iterations of PSI-BLAST only retrieved culicine and Culicoides proteins, indicating the uniqueness of this protein family. No salivary anopheline sequences similar to the 41-kDa family have been reported, including all ENSEMBL-predicted An. gambiæ proteins deposited at the National Center for Biotechnology Information (NCBI) (which contains all proteome versions released by ENSEMBL). EST coding for the two alleles in Æ. ægypti are significantly underrepresented in the non-SG libraries; RT-PCR suggests ubiquitous expression of one of the alleles, although only very faint bands were detected even after 35 cycles of amplification (Figure 5). The function of any member of this protein family is unknown.
30-kDa GE-rich family
This acidic, Gly/Glu-rich protein family is abundantly expressed in adult female mosquito SG, where they appear to be involved in allergic reactions to mosquito bites . In Æ. ægypti, two proteins of this family have been previously reported, and we now report two additional splice variants and alleles. Evidence for expression was found in bands labeled 30 ag (for gi|14423642 and gi|18568322) in the 2D gel experiment shown in Figure 4. The two proteins are coded by an inverted tandem repeat in supercontig1.464 separated by only 363 base pairs. The sialotranscriptome is significantly overrepresented in EST coding for this protein family, indicating it is salivary specific. RT-PCR confirms the female SG specificity of these transcripts. Based on the public sequences available, it appears that in anopheline mosquitoes (An. gambiæ, An. stephensi, An. albimanus, An. dirus), only one polymorphic gene exists for this protein family per genome.
Other mosquito-or Diptera-specific peptides
Additional File 2 also includes 14 additional polypeptides, one of which is an allele, showing sequence similarities to putative proteins from other hematophagous Diptera including, in a few cases, some weak similarities to Drosophila; 13 of these are novel. Among this class of polypeptides, nine were analyzed by RT-PCR (Figure 5 and Table 2), and seven are significantly underrepresented in the non-sialotranscriptomes, as follows: AE-212, which is similar to Drosophila and Culicoides proteins of unknown function and was ubiquitously expressed by RT-PCR experiments; two alleles (AE-165 and AE-163) coding for basic (pI = 9.8) 29-kDa proteins containing 4 putative galactosylated Ser/Thr and similar to Culex and Culicoides salivary proteins; three polypeptides whose expression appeared gland specific, as suggested by RT-PCR (AE-196, which is similar to the An. gambiae gSG8 salivary protein; gi|61742023, which is similar to tryptophan-rich salivary proteins of Culex; and AE-209, similar to another Culex salivary protein). AE-225 (ubiquitously expressed by RT-PCR experiments), is weakly similar to proteins varying in size from 150–180aa residues from Anopheles and Drosophila. Two additional transcripts, AE-937 and AE-752, were expressed solely in the adult female SG by RT-PCR. The function of these proteins remains to be investigated.
Genes belonging to protein families found to date only in Ædes genus
Nineteen genes were found expressed in the sialotranscriptome of Æ. ægypti coding for polypeptide families known only in the Ædes genus, as follows:
Two single exon genes separated by ~20 kb in supercontig1.15 code for proteins with signal peptides and mature mass of 62–63 kDa. Transcripts for these genes are significantly overrepresented in the sialotranscriptome and are shown to be adult female SG specific by RT-PCR (Figure 5 and Table 2). They are similar to homologous salivary protein sequences seen in Ae. albopictus  and, to a much smaller degree, to rhoptry proteins of Plasmodium. Repeated Leu and Glu residues provide similarities to myosin, indicating this protein family may be involved in adhesion phenomena. Their uniqueness among metazoan and single-exon structure indicates possible horizontal acquisition of this gene family in Ædes. Both genes are abundantly expressed in the SG as evidenced by bands labeled 62 k by 2D gel electrophoresis MS/MS (Figure 4).
34-kDa gene family
Two genes coding for proteins of ~30.5-kDa (not to be confused with the 30 kDa/GE-rich protein family) are found as a tandem repeat on supercontig1.280. The gene coding for gi|61742033 is abundantly expressed as evidenced by MS/MS of tryptic digested band labeled 30.5 in Figure 4. These proteins are similar only to homologues found in Ae. albopictus . Both genes are significantly overtranscribed in the sialotranscriptome when compared with other transcriptomes. RT-PCR indicates enrichment in the female SG or exclusive expression in the same organ.
Two genes having 80% sequence similarity and coding for mature peptides of 8.5 and 9.5 kDa are found as a tandem repeat in supercontig1.18. They are similar only to salivary peptides of Ae. albopictus. Both genes are significantly overexpressed in sialotranscriptomes. RT-PCR suggests that these genes occur in both male and female SG.
Other salivary polypeptides
Additional File 2 lists an additional 11 full-length transcripts originating from 10 genes coding for proteins found to date only in the genus Ædes. Six of these genes have overrepresentation in the sialotranscriptome, as follows: AE-376, AE-156, gi|18568314, gi|18568282, AE-211, and AE-214. RT-PCR in 10 of the 11 transcripts show enrichment or female specificity for 5 transcripts and ubiquitous expression for 2 genes, while 3 appear to be transcribed in both male and female SG (Figure 5, Table 2, and Additional File 2). Their function is unknown.
Proteins of possible housekeeping function
Function possibly predicted
Transporter function and storage proteins
Being a secretory organ, mosquito SG are involved in active ion and water transport associated with their function. V-ATPases are generic 'batteries' that generate a proton gradient across membranes that can be coupled with ion exchangers and are used in eukaryotic cells for transport purposes . This multi-subunit enzyme complex has been extensively studied in insects, in particular in Lepidotera larvæ midgut  and in the malpighian fluid transport in mosquitoes . A role for V-ATPases in mosquito SG secretion has been previously proposed . Additional File 2 reports 22 gene products including 9 subunits of the V-ATPase complex, 3 aquaporins (water channels), 2 chloride channels, and the enzyme carbonic anhydrase, which is associated with proton transport in epithelia to speed intracellular pH regulation via the CO2+H2O↔bicarbonate + H+ reaction.
Probable signal transduction function
Thirty-eight proteins, 35 of which are novel, are described in Additional File 2 as possibly associated with signal transduction events. Included are four proteins associated with inhibition of apoptosis, four enzymes associated with juvenile hormone metabolism, one associated with ecdyesteroid metabolism, and a gamma-amino butyric (GABA) receptor-associated protein, in addition to protein kinases and phosphatases.
Nuclear regulation, transcription factors, and transcription machinery
Twelve (all novel) proteins are associated with nuclear function including histones, zinc finger proteins, and proteins associated with cell division. Additionally, we found 9 possible transcription factors and 21 proteins involved in the transcription machinery, only one of which has been previously reported (Additional File 2).
Ribosomal proteins and other proteins associated with the translation machinery
Being an organ involved in abundant protein synthesis, it is not surprising that many ribosomal and other translation associated proteins were identified. Additional File 2 lists 75 ribosomal proteins, 65 of which are novel for Æ. ægypti. Twelve of these ribosomal proteins, although also found in other transcriptomes, are significantly overexpressed in the sialotranscriptome. These are AE-155, AE-198A, AE-430, AE-411, AE-288, AE-200A, AET-3179, AET-2065, gi|78214568, gi|11762110, AET-3168, and AET-3303.
Eighteen proteins (14 novel) were possibly associated with the translation machinery, including elongation factors, tRNA synthases, and translation initiation factors. Elongation factors 2, 2 alpha, and 2 beta were significantly overexpressed in the SG.
Protein modification and protein export machinery
Forty-four proteins (43 novel) were possibly associated with the protein modification machinery including enzymes associated with proline isomerization, disulfide bridge formation, glycosylation, and several chaperones. Thirty-six proteins (35 novel) are possibly associated with the protein export machinery, including signal peptidase complex, endoplasmic reticulum, Golgi, and vacuole proteins. The putative cargo transport protein EMP24 is overexpressed in the sialotranscriptome, although also found in other nonsalivary libraries. Evidence for expression of a protein disulphide isomerase was found by MS/MS results of the tryptic digestion of band labeled PDI in Figure 4.
One peroxiredoxin, one thioredoxin, two superoxide dismutases, one cytochrome P-450 enzyme, and one truncated catalase are among ten proteins associated with oxidant metabolism (eight of which are novel). The cytochrome P-450 enzyme has a signal peptide indicative of secretion and is a member of the CYP4 family (based on the nomenclature of related proteins), but it is included as a possible housekeeping function due to its high similarity to other insect enzymes and because these enzymes normally need an associated reductase driven by NADPH+, which is normally only found intracellularly. Members of the CYP4 family can be found in peroxisomes, where they can be associated to arachidonic acid or eicosanoid reactions . Of interest, AET-6749 is similar to a 40-kDa farnesylated protein associated with peroxisomes, indicating the presence of this organelle in Ædes SG and the possible reason for the signal peptide that may be needed for directing this enzyme to the peroxisome.
Proteasome and lysosomal machinery
Seventeen proteins (16 novel) were associated with the proteasome machinery, including several proteasome subunits and ubiquitin-related enzymes. Two previously described lysosomal enzymes are also listed in Additional File 2.
Forty-three proteins associated with nt, aa, carbohydrate, lipid, and heme metabolism or transport are described. AET-12468 is similar to enzymes annotated as kynurenine formamidase  and has a KOG motif indicative of this enzyme ; 9 transcripts were found among the 15,625 salivary EST but only 6 in the 217,296 nonsalivary EST, indicating this enzyme is overexpressed in the SG of Ædes. Kynurenine formamidase is a key enzyme in the degradation of tryptophan, producing L-kynurenine from N-formyl-kynurenine, the product of the action of L-tryptophan:oxygen 2,3-oxidoreductase on tryptophan, and a precursor to xanthurenic acid, which has been described in the SG of An. stephensi . Xanthurenic acid has also been reported as the mosquito-derived gametocyte exflagellation factor of Plasmodium [155, 156]. The presence of xanthurenic acid in Ædes saliva remains to be demonstrated, although a recent report indicates that Ædes mosquitoes deficient in the production of xanthurenic acid sustain normal P. gallinaceum development .
Fifty-four enzymes (51 novel) are presented as involved in energy metabolism. Most of these are mitochondrial constituents, a few of which are overrepresented in the salivary libraries compared with the other libraries, perhaps due to the larger-than average-salivary metabolism associated with protein synthesis and secretion. Evidence for expression of the alpha and beta subunits of the F0F1-type ATP synthase were found by MS/MS data obtained from bands labeled F0F1α and F0F1β on the 2D gel (Figure 4) indicating their abundant expression.
Cytoskeletal, adhesion, and extracellular matrix proteins
Fifteen proteins (14 are novel to Ædes) are associated with cytoskeletal, intercellular adhesion, or extracellular matrix functions, including actins, dynactin, tubulins and annexins, and the basal lamina protein named as SGS1, which was found to be a SG receptor for P. gallinaceum sporozoites . Notably, this protein family has homologues only in An. gambiæ, where it was abundantly expressed in the SG of adult females  and in bacteria, the most closely related protein outside Anopheles being from the Wolbachia [12, 158]. Four such large proteins (~200 kDa) were described in Anopheles, all intronless and contained in a tandem repeat on chromosome arm 3R, while six have been identified in Ædes, also intronless, and consistent with their horizontal acquisition from a Wolbachia bacteria. For more details on this protein family, see reference .
Probably housekeeping, function unknown
Fifty-nine proteins are described (all novel to Ædes) that are conserved with other organisms, thus characterizing the large group of 'conserved hypothetical' proteins  or are just hypothetical proteins with no similarities to other known proteins (five cases only). Two of the conserved hypothetical proteins are clearly membrane proteins of unknown function. Several members of this group are significantly overexpressed in the SG when EST's in the sialotranscriptome are compared with the remaining transcripts.
Using high-throughput transcriptome analysis, we significantly expanded the Æ. ægypti SG transcript repertoire. A total of 614 transcripts was identified, 573 of which are new, and mostly full length. A subset of 136 transcripts was identified as possibly SG specific, 97 of which are novel. Analysis of tissue-specific transcription of selected genes revealed at least 31 genes whose expression is specific or enriched in female SG, whereas 24 additional genes were expressed in female SG and in males but not in other female tissues. Most of the 55 proteins coded by these transcripts have no known function and represent high-priority candidates for expression and functional analysis as antihemostatic or antimicrobial agents. This catalogue makes Æ. ægypti the mosquito vector for which the most complete salivary transcriptome is available. We hope that this updated catalogue will help our continuing effort of understanding the evolution of blood sucking in vector arthropods and the discovery of novel pharmacologically active compounds.
An unexpected finding of this work was the occurrence of four protein families specific to SG that were probably a product of horizontal transfer from prokaryotic organisms to mosquitoes. Previously, the SGS family was shown to localize specifically in the basal surface of SG cells and may function as a Plasmodium receptor . Here we identify three new families of salivary and possibly secreted proteins (62, 56, and 34 kD) characterized by having uniexonic genes and PSI-BLAST retrieval of only salivary proteins of hematophagous Diptera and bacterial proteins. Although horizontal gene transfer is common in prokaryotic organisms, it is a relatively rare finding in eukaryotes . To the extent that these genes are really of bacterial origin, it may emphasize the unusual paths of SG gene evolution in the quest of hematophagous animals to obtain their 'perfect' potion that allows disarming of the complex host pathways of inflammation and hemostasis that would otherwise disrupt blood feeding.
Two laboratory colonies were used in this work, one at Dr. Ribeiro's laboratory at the National Institutes of Health (NIH), and the other in Dr. Wikel's laboratory at the University of Connecticut Health Center (UCHC). Both mosquito colonies were the Liverpool/blackeye strain of Æ. ægypti. Insectary rooms were kept at 26°C ± 0.5°C (NIH) or 27°C ± 0.5°C (UCHC), with a relative humidity of 70% to 75% and a 16 h:8 h light:dark photoperiod. Adult female mosquitoes used in the experiments were 0–7 days old, took no blood meals, and were maintained on a diet of 10% Karo syrup solution (NIH) or raisins (UCHC).
At the NIH, SG from adult female mosquitoes were dissected and transferred to 20μl Hepes saline (HS; NaCl 0.15 M, 10 mM Hepes, pH7.0) in 1.5 ml polypropylene vials in groups of 20 pairs of glands in 20 μl of HS or as individual glands in 10μl of HS. SG were kept at -75°C until needed. At UCHC, adult female mosquitoes were primed for blood feeding before dissecting out their SG by placing a human hand close to the mosquito cage for 3–5 min (without letting the mosquitoes probe). SG were dissected and placed into a solution of 75% RNA-Later (Ambion) 25% 1 × PBS (RNAse free) and stored in 100% RNA-Later at -20°C for isolating polyA+ RNA.
Æ. ægypti SG mRNA was isolated from 80SG pairs (NIH) or 110 pairs (UCHC) from adult females at days 1 and 2(NIH) or 1–4 (UCHC) after emergence using the Micro-FastTrack mRNA isolation kit (Invitrogen) (NIH) or the Oligotex™ direct mRNA isolation kit (Qiagen) (UCHC). The PCR-based cDNA library was made following the instructions for the SMART (s witching m echanism a t 5' end of R NA t ranscript) cDNA library construction kit (Clontech). This kit provided a method for producing high-quality, full-length cDNA libraries from nanogram quantities of polyA+ or total RNA. It utilizes a specially designed oligonucleotide named SMARTIV™ in the first-strand synthesis to generate high yields of full-length, double-stranded cDNA. Æ. ægypti SG polyA+ RNA (300 ng) was used for reverse transcription to cDNA using PowerScript reverse transcriptase (Clontech), the SMARTIV oligonucleotide, and the CDS III/3' primer (Clontech). The reaction was carried out at 42°C for 1 h. Second-strand synthesis was performed by a long-distance PCR-based protocol using the 5' PCR primer and the CDS III/3' primer as sense and antisense primers, respectively. These two primers also create Sfi1A and B restriction enzyme sites at the end of nascent cDNA. Advantage™ Taq polymerase mix (Clontech) was used to carry out the long-distance PCR reaction on a Perkin Elmer GeneAmp® PCR system9700 (Perkin Elmer Corp.). The PCR conditions were: 95°C for 20s; 24 cycles of 95°C for 5s, 68°C for 6 min. A small portion of the cDNA was analyzed on a 1.1% agarose/EtBr (0.1 μg/ml) gel to check for the quality and range of the cDNA synthesized. Double-stranded cDNA was immediately treated with proteinaseK (0.8 μg/ml) at 45°C for 20 min, extracted with phenol:chloroform:iso-amyl alcohol mixture, and precipitated using sodium acetate (200 mM), glycogen (0.12 μg/ml), and 95% ethanol. The clean double-stranded cDNA was then digested with SfiI restriction enzyme at 50°C for 2 h followed by size fractionation on a ChromaSpin-400 drip column (Clontech). The profiles of the fractions were checked on a 1.1% agarose/EtBr (0.1 μg/ml), and fractions containing cDNA of more than 400 bp were pooled and concentrated by precipitation. The cDNA were then ligated into a λ TriplEx2 vector (Clontech), and the resulting ligation mixture was packaged using GigaPack® IIIPlus packaging extract (Stratagene) according to the manufacturer's instructions. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech). The percentage of recombinant clones was determined by performing a blue-white selection screening on LB/MgSO4 plates containing X-gal/IPTG. Recombinants were also determined by PCR, using vector primers (5' λ TriplEx2 and 3' λ TriplEx2 sequencing primers) flanking the inserted cDNA and visualizing the products on a 1.1% agarose/EtBr gel.
Sequencing of the Æ. ægypti cDNA Library
The Æ. ægypti SG cDNA library was plated on LB/MgSO4 plates containing X-gal/IPTG, to an average of 250 plaques per 150 mm Petri plate. Recombinant (white) plaques were randomly picked up and transferred to 96-well MICROTEST™ U-bottom plates (BD BioSciences) containing 100 μls of SM buffer (0.1 M NaCl, 0.01 M MgSO4, 0.035 M Tris-HCl [pH7.5], 0.01% gelatin) per well. The plates were covered and placed on a gyrating shaker for 30 min at room temperature. The phage suspension was either immediately used for PCR or stored at 4°C for future use.
To amplify the cDNA using a PCR reaction, 4 μl of the phage sample was used as a template. The primers were sequences from the λ TriplEx2 vector and named pTEx2 5 seq (5' -TCCGAGATCTGGACGAGC-3' ) and pTEx2 3LD (5' -atacgactcactatagggcgaa ttggc-3' ), positioned at the 5' end and the 3' end of the cDNA insert, respectively. The reaction was carried out in 96-well flexible PCR plates (Fisher Scientific) using TaKaRa EX Taq polymerase (TAKARA; Mirus Bio), on a Perkin Elmer GeneAmp® PCR system9700 (Perkin Elmer Corp.). The PCR conditions were: 1 hold of 95°C for 3 min, 25 cycles of 95°C for 1 min, 61°C for 30s, 72°C for 2 min. The amplified products were analyzed on a 1.5% agarose/EtBr gel. cDNA library clones (1100 clones) were PCR amplified, and those showing a single band were selected for sequencing. Approximately 200–250 ng of each PCR product was transferred to Thermo-Fast 96-well PCR paltes (ABgene Corp.) and frozen at -20°C. Sequencing of the Wikel's laboratory library was performed by Agencourt Bioscience Corp., and a total of 1,017 cDNA library clones was sequenced. The library constructed in Ribeiro's lab was sequenced locally using an 8 capillary CEQ 2000 DNA sequencing instrument (Beckman Coulter, Inc) to provide 2,759 sequences.
2D gel electrophoresis was performed using ZOOM IPGRunner System (Invitrogen) under manufacturer's recommended running conditions. Briefly, approximately 50 μg of sample proteins (approximately 15 pairs of SG) were solubilized with 155 μl rehydration buffer (7 M urea, 2 M thiourea, 2% CHAPS, 20 mM DTT, 0.5% carrier ampholytes, pH3-10). The samples were absorbed by rehydration ZOOM strips (7 cm; pH3-10NL) overnight at room temperature and then focused under manufacturer's recommended conditions. The focused IPG strips were reduced/alkylated/equilibrated with reducing and then alkylation reagents dissolved in the sample buffer. The strips were then applied onto NuPAGE 4–12% Bis-Tris ZOOM gels (Invitrogen). The gels were run under MOPS buffer and stained with SeeBlue staining solution (Bio-Rad). A total of 75 spots were selected for tryptic digestion, based on their staining intensity. The gel picture and 23 protein bands matched to Æ. ægypti proteins are shown in Figure 4.
Protein identification by mass spectrometry
Protein identification of 2Dgel-separated proteins was performed on reduced and alkylated trypsin-digested samples prepared by standard mass spectrometry protocols. Tryptic digests were analyzed by coupling the Nanomate (Advion BioSciences) – an automated chip-based nano-electrospray interface source – to a quadrupole time-of-flight mass spectrometer, QStarXL MS/MS System (Applied Biosystems/Sciex). Computer-controlled, data-dependent automated switching to MS/MS provided peptide sequence information. AnalystQS software (Applied Biosystems/Sciex) was used for data acquisition. Data processing and databank searching were performed with Mascot software (Matrix Science). The NR protein database from the NCBI, National Library of Medicine, NIH, was used for the search analysis, as was a protein database generated during the course of this work.
Bioinformatic tools and procedures
EST were trimmed of primer and vector sequences, clusterized, and compared with other databases as described before . The BLAST tool , CAP3 assembler , ClustalW , and Treeview software  were used to compare, assemble, and align sequences and to visualize alignments. Phylogenetic analysis and statistical neighbor-joining bootstrap tests of the phylogenies were also done with the Mega3 package . For functional annotation of the transcripts we used the tool blastx  to compare the nt sequences with the NCBI NR protein database of the NCBI and to the Gene Ontology (GO) database . The tool rpsblast  was used to search for conserved protein domains in the Pfam , Smart , Kog , and conserved domains (CDD) databases . We have also compared the transcripts with other subsets of mitochondrial and rRNA nt sequences downloaded from NCBI and to several organism proteomes downloaded from NCBI (yeast), Flybase (D. melanogaster), or ENSEMBL (An. gambiæ). Segments of the three-frame translations of the EST (as the libraries were unidirectional, we did not use six-frame translations) starting with a methionine in the first 100 predicted aa – or the predicted protein translation, in the case of complete coding sequences – were submitted to the SignalP server  to help identify translation products that could be secreted. O-glycosylation sites on the proteins were predicted with the program NetOGlyc . Functional annotation of the transcripts was based on all the comparisons above. Following inspection of all results, transcripts were classified as either (S)ecretory, (H)ousekeeping, or of (U)nknown function, with further subdivisions based on function and/or protein families. To map the EST and contigs in the genome, blastn was used . To speed the program, each genomic fasta file was broken into 30-kb fragments with 5 kb from previous sequence. For visualization of EST on the Æ. ægypti genome, we used the Artemis tool  after transforming the blastn output to a file compatible to Artemis using a program written in Visual Basic.
To compare the EST frequency in Æ. ægypti salivary cDNA libraries with EST frequency in other libraries whose mRNA derive from other sources (downloaded from the NCBI EST database DBEST), all available EST from Æ. ægypti plus the EST set from a EST hemocyte library from DrBruce Christensen's laboratory  plus our own salivary EST set were pooled to obtain a total of 232,921 EST; these were assembled as described above to create a searchable annotated database of 28,458 contigs and singletons, which is available for browsing at Anobase . The combined EST database thus derives from 29 different EST libraries, 2 of which are from SG of adult female mosquitoes (4,040 from Ribeiro/Wikel laboratories, and 11,585 from Dr. Sergio Verjovski's laboratory); the remainder are from different organs or whole organisms at different developmental stages, or from adult mosquitoes infected or not with different pathogens. Details of these libraries are available at the EST dataset website . From each of these 28,458 contigs, we determined the EST contribution from each of the 29 libraries to the final assembled contigs, thus obtaining for each contig the total salivary and nonsalivary contribution. A χ2test was applied to the data set to determine whether a salivary contribution was above or below the null hypothesis of no differential library contribution when the expected EST frequency was above 5, as indicated for the correct use of the test. When the Pvalue was below 0.05, we considered the deviation of equal EST distribution among salivary and remaining libraries as significant.
RT-PCR expression analysis
For RT-PCR analysis, SG were dissected from adult females 1 to 5 days after emergence and stored at -80°C. Total RNA was extracted from female glands, carcasses (i.e. adult females with SG removed), and adult males using the TRIZOL reagent (Invitrogen).
Approximately 50 ng RNAse-free DNase-treated total RNA (Invitrogen) was used for the RT-PCR amplification by the SuperScript one-step RT-PCR system (Invitrogen) according to manufacturer's instructions. Typically, reverse transcription (50°C, 30 min) and heat inactivation of the reverse transcriptase (94°C, 2 min) were followed by 30 PCR cycles: 30s at 94°C, 30s at 55°C, 1 min. at 72°C; 25 cycles were used for the amplification of the ribosomal protein S5 mRNA (rpS5) to keep the reaction below saturation levels and to allow reliable normalization. For some clones (gi|94468620, gi|94468350, gi|94468634, and gi|42632615), 35 cycles of amplification were needed to obtain detectable bands. The oligonucleotide primers used for rpS5 amplification were: rpS5-F, 5' -ATTACATCGCCGTCAAGG AG-3' , and rpS5-R, 5' -TCATC ATCAGCGAGTTGGTC-3'. The sequence of the other oligonucleotide primers is available as Supplemental Material. Amplification reactions were analyzed on 1.2% agarose gels. Each sample was analyzed by RT-PCR two to three times using independent batches of total RNA.
expressed sequence tag
reverse transcriptase polymerase chain reaction
sodium dodecyl sulfate/polyacrylamide gel electrophoresis
switching mechanism at 5' end of RNA transcript
This work was supported in part by grants from the European Union to Mario Coluzzi and B.A. (BioMalPar N503578); by the Intramural Research Program of the National Institute of Allergy and Infectious Diseases, National Institutes of Health to JMCR; and by award number DAMD17-03-1-005 from the United States Army Medical Research and Materiel Command to S.K.W. We thank NIAID intramural editor Brenda Rae Marshall and Chuong Huynh (NCBI) for assistance.We are grateful to the NIAID Research Technology Branch under the direction of Dr. R.J. Hohman for the performance of the 2D Gel electrophoresis and mass spectrometry experiments.
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- AE-389 blast comparison. [http://www.ncbi.nlm.nih.gov/sutils/blink.cgi?pid=94468538]
- TIL domain on AE-389. [http://www.ncbi.nlm.nih.gov/projects/omes/Ae_aegypti_sialome_2006/Sup_table_2/links/CDD/AE-389-CDD.txt]
- TIL domain peptide in An. stephensi. [http://www.ncbi.nlm.nih.gov/Structure/cdd/wrpsb.cgi?INPUT_TYPE=precalc&SEQUENCE=27372905]
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- Single exon structure of 56 kDa protein. [http://www.ncbi.nlm.nih.gov/projects/omes/Ae_aegypti_sialome_2006/Sup_table_2/links/AEGFRAG/18568292-AEGFRAG.txt]
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- 41 kDa family mismatch. [http://www.ncbi.nlm.nih.gov/sutils/blink.cgi?pid=61742035]
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- 29 kDa family transcripts. [http://www.ncbi.nlm.nih.gov/projects/omes/Ae_aegypti_sialome_2006/Sup_table_2/links/ae-comp-ext95-60-Sim-CLTL7.txt]
- Non-salivary 29 kDa family member. [http://www.ncbi.nlm.nih.gov/projects/omes/Ae_aegypti_sialome_2006/Sup_table_2/links/AE-EST/AE-236A-AE-EST.txt]
- PSIBlast of 29 kDa family. [http://www.ncbi.nlm.nih.gov/projects/omes/Ae_aegypti_sialome_2006/Sup_table_2/links/PSIB/29kda-psi.rtf]
- AE-212 blast comparison. [http://www.ncbi.nlm.nih.gov/projects/omes/Ae_aegypti_sialome_2006/Sup_table_2/links/NR/AE-212-NR.txt]
- AE-165 sequence. [http://www.ncbi.nlm.nih.gov/sutils/blink.cgi?pid=94468362]
- AE-163 sequence. [http://www.ncbi.nlm.nih.gov/sutils/blink.cgi?pid=94468360]
- Blast comparison of AE-165. [http://www.ncbi.nlm.nih.gov/projects/omes/Ae_aegypti_sialome_2006/Sup_table_2/links/NR/AE-165-NR.txt]
- Aedes protein similar to An. gambiae sSG8. [http://www.ncbi.nlm.nih.gov/projects/omes/Ae_aegypti_sialome_2006/Sup_table_2/links/NR/AE-196-NR.txt]
- Aedes protein similar to W rich Culex protein. [http://www.ncbi.nlm.nih.gov/projects/omes/Ae_aegypti_sialome_2006/Sup_table_2/links/NR/61742023-NR.txt]
- Aedes protein similar to Culex salivary protein. [http://www.ncbi.nlm.nih.gov/projects/omes/Ae_aegypti_sialome_2006/Sup_table_2/links/NR/AE-209-NR.txt]
- Aedes protein similar to Anopheles protein. [http://www.ncbi.nlm.nih.gov/projects/omes/Ae_aegypti_sialome_2006/Sup_table_2/links/AGPROT/AE-225-AGPROT.txt]
- Similar to Drosophila peptide. [http://www.ncbi.nlm.nih.gov/projects/omes/Ae_aegypti_sialome_2006/Sup_table_2/links/DMPROT/AE-225-DMPROT.txt]
- Single exon structure of 62 kDa protein . [http://www.ncbi.nlm.nih.gov/projects/omes/Ae_aegypti_sialome_2006/Sup_table_2/links/AEGFRAG/18568302-AEGFRAG.txt]
- 62 kDa homologue in Ae. albopictus. [http://www.ncbi.nlm.nih.gov/sutils/blink.cgi?pid=18568300]
- 62 kDa family similarity to myosin. [http://www.ncbi.nlm.nih.gov/projects/omes/Ae_aegypti_sialome_2006/Sup_table_2/links/KOG/18568302-KOG.txt]
- Two exon structure of a 34 kDa family member. [http://www.ncbi.nlm.nih.gov/projects/omes/Ae_aegypti_sialome_2006/Sup_table_2/links/AEGFRAG/AEA-286-AEGFRAG.txt]
- Single exon structure of 34 kDa family members. [http://www.ncbi.nlm.nih.gov/projects/omes/Ae_aegypti_sialome_2006/Sup_table_2/links/AEGFRAG/AEA-105-AEGFRAG.txt]
- 34 kDa family in Ae. albopictus. [http://www.ncbi.nlm.nih.gov/projects/omes/Ae_aegypti_sialome_2006/Sup_table_2/links/NR/18568296-NR.txt]
- 30.5 kDa family member 1 in Aedes albopictus. [http://www.ncbi.nlm.nih.gov/projects/omes/Ae_aegypti_sialome_2006/Sup_table_2/links/NR/AE-260-NR.txt]
- Second 30.5 kDa member in Aedes albopictus. [http://www.ncbi.nlm.nih.gov/projects/omes/Ae_aegypti_sialome_2006/Sup_table_2/links/NR/61742033-NR.txt]
- 9 kDa family. [http://www.ncbi.nlm.nih.gov/projects/omes/Ae_aegypti_sialome_2006/Sup_table_2/links/ae-comp-ext70-60-Sim-CLTL21.txt]
- 9 kDa family in Aedes albopictus. [http://www.ncbi.nlm.nih.gov/projects/omes/Ae_aegypti_sialome_2006/Sup_table_2/links/NR/AE-216-NR.txt]
- AE-376 sequence. [http://www.ncbi.nlm.nih.gov/sutils/blink.cgi?pid=94468530]
- AE-156 blast comparison. [http://www.ncbi.nlm.nih.gov/sutils/blink.cgi?pid=94468356]
- gi|18568314 blast comparison. [http://www.ncbi.nlm.nih.gov/sutils/blink.cgi?pid=18568314]
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- AE-214 sequence. [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=protein&val=94468394]
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