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Table 3 A subset of genes down-regulated in 5-AzaC treated E. histolytica HM-1:IMSS parasites as compared to untreated controls.

From: Growth of the protozoan parasite Entamoeba histolytica in 5-azacytidine has limited effects on parasite gene expression

Probe set

Baseline expression

Fold-change

p-value

Annotation

Potentially virulence associated

2.m00545_at* £

4.12

0.27

0.033

cysteine proteinase, putative (GO:0006508)

52.m00148_at

0.96

0.46

0.018

lysozyme, putative

1.m00663_at

6.21

0.36

0.006

myosin calcium-binding light chain, putative (GO:0005509)

Protein kinases

35.m00259_at

1.76

0.48

0.013

protein kinase, putative (GO:0005524)

67.m00091_x_at£

3.42

0.37

0.033

protein kinase, putative (GO:0005524)

99.m00190_s_at

0.39

0.46

0.040

protein kinase, putative (GO:0006468)

466.m00033_x_at

0.51

0.47

0.003

protein kinase, putative (GO:0005524)

264.m00068_at

2.29

0.44

0.029

protein kinase, putative (GO:0005524)

GTPases

106.m00140_at

2.39

0.48

0.025

Rap Ran GTPase activating protein, putative

2.m00606_x_at

0.20

0.36

0.005

Rab family GTPase (GO:0005524)

226.m00092_at*

0.34

0.44

0.008

Rab family GTPase

30.m00257_at

4.28

0.50

0.023

Rab family GTPase (GO:0005524)

34.m00273_x_at

3.22

0.43

0.040

Rab family GTPase (GO:0005524)

71.m00153_x_at£

2.21

0.47

0.002

Rho family GTPase (GO:0005525)

8.m00366_s_ata

1.15

0.46

0.021

Rho GTPase activating protein, putative

171.m00089_at

4.88

0.35

0.044

GTPase activating protein, putative (GO:0003924)

Genes with decreased expression in E. histolytica (Rahman) compared to E. histolytica (HM-1:IMSS)

108.m00122_at

32.12

0.21

0.036

hypothetical protein

136.m00107_at

1.20

0.38

0.030

hypothetical protein

22.m00298_at

4.12

0.43

0.002

hypothetical protein

297.m00061_at

5.00

0.35

0.010

hypothetical protein

32.m00239_at

0.93

0.46

0.039

N-acetylglucosaminyl transferase, putative

37.m00215_at

1.90

0.46

0.035

hypothetical protein

442.m00023_x_at

0.82

0.44

0.006

hypothetical protein

460.m00024_s_atb

2.18

0.27

0.015

hypothetical protein

Genes overlap with day-1 & day-29 significantly up-regulated genes from in vivo mouse model (Gilchrist et al , 2006) £

15.m00302_at

4.05

0.41

0.030

hypothetical protein

2.m00545_at*

4.12

0.27

0.033

cysteine proteinase, putative (GO:0006508)

67.m00091_x_at

3.42

0.37

0.033

protein kinase, putative (GO:0005524)

71.m00153_x_at

2.21

0.47

0.002

Rho family GTPase (GO:0005525)

Miscellaneous

338.m00049_s_atc

4.30

0.42

0.020

LIM domain protein (GO:0008270)

10.m00349_x_at

4.30

0.44

0.024

paxillin, putative (GO:0008270)

35.m00253_at

1.74

0.26

0.036

iron-sulfur flavoprotein, putative (GO:0006118)

646.m00021_s_at

0.36

0.40

0.009

iron-sulfur flavoprotein, putative

90.m00179_at

1.31

0.46

0.044

amino acid transporter, putative (GO:0006865)

139.m00118_at

3.90

0.44

0.048

histone H3, putative (GO:0005634)

  1. a8.m00366_s_at also represents 58.m00170 (Rho GTPase activating protein, putative)
  2. b460.m00024_s_at also represents 353.m00048 (hypothetical protein) and 353.m00049 (hypothetical protein)
  3. c338.m00049_s_at also represents 321.m00056 (LIM domain protein)
  4. The probe set, baseline expression value in untreated E. histolytica HM-1:IMSS trophozoites, fold change in 5-AzaC treated parasites, p-value, and gene annotation are shown. Genes are clustered according to functional categories. Genes in bold are those for which RT-PCR confirmation was performed. Genes with normalized expression values >0.20 can routinely be detected by RT-PCR. Genes marked by * are those for which bisulfite sequencing was performed. Genes that were significantly upregulated in E. histolytica HM-1:IMSS trophozoites passed through mice are obtained from Gilchrist et al [27] and are indicated by £. For annotations, GO IDs are given where available and are provided within parentheses.
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BMC Genomics

ISSN: 1471-2164

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