Figure 3

Lower level of expression of SAMD9 and SAMD9L in neoplasia. A. Validation curves for the primer sets of SAMD9 and 28S rRNA, and real time quantitative PCR of SAMD9. SAMD9 and 28S rRNA have similar amplification efficiency since the slope was very similar for both curves. SAMD9 was expressed at a lower level in aggressive fibromatosis (AF) tumors compared to normal fibrous tissues from same patients. B. Validation curves for the primer sets of SAMD9L and 28S rRNA, and real time quantitative PCR of SAMD9L. SAMD9L and 28S rRNA have similar amplification efficiency since the slope was very similar for both curves. SAMD9L was expressed at a lower level in breast cancers compared to normal breast epithelial tissues from same patients.C. Semi-quantitative RT-PCR of SAMD9. SAMD9 was under-expressed in some cases of colon cancer compared to the normal colon mucosa from same patients. Real time PCR data showed no significant difference in expression level between breast and colon cancer and normal controls as a group. D. There was no significant difference in expression of SAMD9L between aggressive fibromatosis samples and normal controls tissues.