Figure 5

Real-time PCR analysis of the expression of candidate genes in hen tissues. Total RNA was isolated from whole ovary (O), spleen (Sp), intestine (I), gizzard (G), liver (L), heart (H), skin (S), brain (B), pectoralis muscle (M), lung (Lu) and pituitary gland (Pg) and real time PCR was performed as described in the Materials and Methods. Ribosomic RNA 18S was used as a reporter gene. The negative control (water) is indicated as (C-). The results represent the means ± SEM. The same letters indicate that differences were not significant. Different letters indicate that differences were significant (p < 0.05).