Table 4 Oligonucleotide primer sequences
From: Search for the genes involved in oocyte maturation and early embryo development in the hen
| Â | Abbreviation | Forward | Reverse | Efficiency | Length (bp) |
|---|---|---|---|---|---|
Real time PCR | btg4 | TTGGGTGTTTTTGGGAGG | AGTGCTTCAACTGCTTCTCAGACC | 1.93 | 187 |
| Â | chkmos | TACTCGTGTGACATCGTGACTGGC | TTGCTGGCAAACATGGTGGC | 2.09 | 177 |
| Â | dazL | TACCCATTCGTCAACAACCTGC | CCCTTTGGAAACACCAGTTCTG | 1.88 | 184 |
| Â | fbox | ACCTGTGCTGGATGATGTTGACC | CAACAAGAGGTATGTGCTTTGCG | 1.81 | 197 |
| Â | hsf1 | ACCCCTATTTCATCCGTGGC | AGTCCATGCTCTCCTGCTTTCC | 1.80 | 165 |
| Â | msh4 | GATTCTCGGAATGGTCACACGC | AGCATCAACAAGTGGCTCCAGG | 2.16 | 105 |
| Â | mtprd | TGAAGATCAAGGTCCAACAACTGC | TTGCTTCCTGAAACCTTTGGC | 1.80 | 180 |
| Â | mcmip | CGTGATGGCAGGAGAAGAAAGC | ATGAGTGAGGAAGGATGGGTGACC | 1.80 | 165 |
| Â | zn finger RIZ | GAGCAAAAGAGTACATCAGAGG | CCATTTGATTCACCTCTTGC | 1.80 | 89 |
| Â | mark3 | AGTGAAAGAACCACTGCTGATAGGC | TTGAAGCGACAGGCGTTCTTT | 1.83 | 91 |
| Â | discs5 | ATGACTGTATGGTGGTTGAGACTGC | CGGCCTTTTGAAAGTATCAGGG | 1.81 | 176 |
| Â | trans fact 20 | TCAAAACTCAGCACCAGCCC | GTGCAGGTTTCTCTTGTCCACC | 1.92 | 178 |
| Â | wee | GGAGAAGAGGGTGAAGACAGAAGC | TGAGCTTCCTGTGAGGAGTTGC | 1.90 | 121 |
| Â | zar1 | GTTTGTTTAGGGCTCTTCCAGGG | TTTACTCGCAGCTTTCCCAGC | 2.20 | 92 |
| Â | ktfn | ACTATTTTTCTCCTCCTGCCTCTGC | AATGCCAAATACAAGCGGGG | 2.05 | 186 |
| Â | zpA | CCTTAAATCCAACAACTCCACAGC | CAGCAAAAATCCCAACAAGAGG | 1.92 | 131 |
| Â | zpC | ACTAGCTCTGCCTTCATCACACCC | GGCAGGTGATGTAGATCAGGTTCC | 1.80 | 109 |
| Â | foxL2 | Govoroun et al. 2004 | Â | 1.83 | Â |
| Â | igf 2 | Heck et al. 2003 | Â | 1.85 | Â |
| Â | cvh | AGTTCCTGGCATCTTTGGGC | AGCGTCCTTTGAGAACTCCTGC | 2.00 | 131 |
| Â | zpD | TCATTGAGACAGGGAGGGAAGC | TCTTCACCACCTGCTCATAGGC | 1.90 | 102 |
| Â | gapdh | TGCTGCCCAGAACATCATCC | ATCAGCAGCAGCCTTCACTACC | 2.05 | 199 |
| Â | beta actin | CAGATGTGGATCAGCAAGCAGG | TTTCATCACAGGGGTGTGGG | 1.88 | 107 |
| Â | ef1 alpha | AGC AGA CTT TGT GAC CTT GCC | TGA CAT GAG ACA GAC GGT TGC | 2.00 | 90 |
in situ hybridization | btg4 | ACGGTCTTCTTCATCACGAGGC | TCTGTAGCACCAGCCTTCATCC | Â | 729 |
| Â | chkmos | CCCTGGCAAAGATGGAAAAGC | CAGAGGGTGATGGCAAAGGAGT | Â | 732 |
| Â | dazL | TGTTTTTAAGTGTGCGGGCG | ACTATTACCAGACATCTGTGTGGGC | Â | 749 |
| Â | fbox | TGTGTTCCTTTACCCCGATTGC | AACTGCTACACTGCTTTCAGTCAGG | Â | 794 |
| Â | zpA | TCATCGCTCCTCTCTTTGTTGG | TTTGCATGTGGGATCTCTGAGC | Â | 773 |
| Â | zpC | TACCGCACGCTCATCAACTACG | ATCAGCTGCAACCTCTTTCCCG | Â | 744 |
| Â | cvh | AGTTCCTGGCATCTTTGGGC | ATATCGAGCATGCGGTCTGC | Â | 792 |
| Â | zpD | TATTTGCTGCTGTTCTCTGCCC | TGGTGCTGCCCTTCTATCTTCA | Â | 740 |