Arsenic-induced changes in the gene expression of lung epithelial L2 cells: implications in carcinogenesis

BMC Genomics

Table 2 Real-time PCR primers of genes verified using real-time PCR

GenBank ID	Gene	Up	Down
NM_012980	MMP11	CACTTTGACTATGACGAGACTTGGA	CCTTAGCTGCTGTGGTGTGTTG
NM_012797	Id1	GAACGTTCTGCTCTACGACATGA	GCAGTATCTCCACCTTGCTCACTT
NM_012812	Cox6a2	TCGCTTAACTGCTGGATGCA	ATTGTGGAAAAGCGTGTGGTT
NM_019370	Ap	GTCTGCTGGCTGTGGTTTTACTAC	TCTTTGGTTGACTTGCTCTTCTTG
NM_017025	Ldha	GCACAAGCAGGTGGTTGACA	CATTATGCTCTCGGCCAAGTCT
AF184983	Gpnmb	ATGACCGGAACTCGTCTGATG	AATGGCAGAGTAGTTGAGGAAATGA
NM_022922	Tpi1	ACTGGAGCGACTTGCAAAGAG	TGGCATTGATAATGTCCACGAA
NM_053291	Pgk1	CAACCAGATAACGAATAACCAAAGG	CCAGGTGGCTCATAAGCACAA
AJ277957	ARL	GCCAGATGACCCTTCACTATTACA	TCTGGATGTGGAACCGAATAAGA
NM_031054	MMP2	GTTTATTTGGCGGACAGTGACA	GGCCTCATACACAGCGTCAAT
NM_031012	Anpep	CTTCTACCGCAGCGAGTACATG	TCATCGAAGCAAGGAAAGGATT

Primers were designed using Primer Express software. Each primer was 20–25 bp long with a Tm of 58–60°C. All the genes were sequences from the Rattus norvegicus. MMP11, Matrix Metalloproteinase 11; Id1, Inhibitor of DNA binding 1, helix-loop-helix protein (splice variation); Cox6a2, cytochrome c oxidase, subunit VIa, polypeptide 2; Ap, alkaline phosphodiesterase; Ldha, lactate dehydrogenase A; Gpnmb, glycoprotein nonmetastatic melanoma protein B; Tpi1, triosephosphate isomerase 1; Pgk1, phosphoglycerate kinase 1; ARL, aldose reductase-like protein; MMP2, matrix metalloproteinase 2 (72 KDa type IV collagenase); Anpep, alanyl (membrane) aminopeptidase.

ISSN: 1471-2164