Annotated ESTs from various tissues of the brown planthopper Nilaparvata lugens: A genomic resource for studying agricultural pests

Table 1 List of the EST database of Nilaparvata lugens

Library	Tissue/sex/stage	Vector used	No. of clones	Average length (bp)	%"N"	No. of clusters
AA	Abdomen, female, 0–1 day	ZAP	609	589.7	0.014	435
AB	Abdomen, female, 0–1 day	ZAP	1027	601.9	0.011	667
AC	Abdomen, male, 0–1 day	ZAP	588	599.7	0.022	493
AD	Abdomen, male, 0–1 day	ZAP	967	594.9	0.102	726
EA	Egg, 0–1.5 day	pGEM	3726	652.0	0.051	2371
EB	Egg, 3–5 day	pGEM	2055	576.3	0.076	941
HA	Head, female, 0–1 day	ZAP	777	520.6	0.018	111
HT	Head-thorax, male, 0–1 day	ZAP	2555	673.9	0.070	1132
MA	Midgut, female, 0–5 day	pGEM	3594	637.5	0.051	1252
MB	Midgut, male, 0–5 day	pGEM	2095	606.4	0.117	847
NA	Whole nymph, 1st instar	pGEM	3269	633.8	0.096	1714
NB	Whole nymph, 4th instar	ZAP	3710	624.7	0.058	1749
OA	Ovary, 0–1 day	ZAP	967	511.5	0.119	689
OB	Ovary, 4–5 day	ZAP	822	601.9	0.028	329
OC	Ovary, 4–5 day	ZAP	2225	604.4	0.079	989
SG	Salivary glands, female, 0–5 day	pGEM	2383	640.8	0.083	723
TA	Testis, 0–1 day	ZAP	1525	575.9	0.045	895
TH	Thorax, 0–1 day	ZAP	4228	693.1	0.029	1657
Total			37122	626.7	0.065	10261

ZAP, lambda ZAP II (pBluescript) phage vector; pGEM, pGEM-t plasmid vector; %"N", percentage of ambiguous nucleotides. Ovary and testis includes the accessory glands of the reproductive organs. Clustering was performed using CLOBB [25]. ESTs are deposited in the DNA databases under accession numbers DB20956–DB858077 (37,122 entries).

ISSN: 1471-2164