Table 3 Parameter settings relaxed for round three in the primer design process.
Primer length, round 3 | 16 – 28 nucleotides |
Product length, round 3 | 80 – 200 nucleotides |
Max. primer melting temperature diff., round 3 | 4.0 ° |
Max. base repeat, round 3 | 4 (e.g., GGGG in a primer is allowed, although not in the 3'-end) |