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Table 1 Novel miRNAs identified from soybean roots

From: Novel and nodulation-regulated microRNAs in soybean roots

miRNA ID

Sequence (5'-3')a

Length

Validationb

gma-MIR1507

UCUCAUUCCAUACAUCGUCUGA

22

mi*

gma-MIR1508

UAGAAAGGGAAAUAGCAGUU

20

N (21 nt)

gma-MIR1509

UUAAUCAAGGAAAUCACGGUCG

22

mi*, N (23 nt)

gma-MIR1510

UGUUGUUUUACCUAUUCCACC

21

N (21 nt)

gma-MIR1511

AACCAGGCUCUGAUACCAUG

20

--

gma-MIR1512

UAACUGAAAAUUCUUAAAGUA

21

--

gma-MIR1513

UGAGAGAAAGCCAUGACUUAC

21

N (21 nt)

gma-MIR1514a

UUCAUUUUUAAAAUAGGCAUU

21

--

gma-MIR1514b

UUCAUUUUUAAAAUAGACAU

20

--

gma-MIR1515

UCAUUUUGCGUGCAAUGAUCU

21

--

gma-MIR1516

CAAAAGAGCUUAUGGCUUGUA

23

--

gma-MIR1517

AGUCUUGGUCAAUGUCGUUCGAAA

24

--

gma-MIR1518

UGUGUUGUAAAGUGAAUAUCA

21

--

gma-MIR1519

UAAGUGUUGCAAAAUAGUCAUU

22

--

gma-MIR1520ac

UAGAACAUGAUACAUGACAGUCA

23

--

gma-MIR1520bc

GUGACAGUCAUCAUUUAAUAAGA

23

--

gma-MIR1520cc

UUCAAUAAGAACGUGACACGUGA

23

--

gma-MIR1520dc

AUCAGAACAUGACACGUGACAA

22

--

gma-MIR1521

CUGUUAAUGGAAAAUGUUGA

20

--

gma-MIR1522

UUUAUUGCUUAAAAUGAAAU

20

--

gma-MIR1523

AUGGGAUAAAUGUGAGCUCA

20

--

gma-MIR1524

CGAGUCCGAGGAAGGAACUCC

21

--

gma-MIR1525

UGGGUUAAUUAAGUUUUUAGU

21

--

gma-MIR1526

CCGGAAGAGGAAAAUUAAGCAA

22

--

gma-MIR1527

UAACUCAACCUUACAAAACC

20

--

gma-MIR1528

AUAGAUUAGAUCAAUAUAUUAGU

23

--

gma-MIR1529

UUAAAGGAAACAAUUAAUCGUUA

23

--

gma-MIR1530

UUUUCACAUAAAUUAAAAUAU

21

--

gma-MIR1531

UCGUCCAUAUGGGAAGACUUGUC

23

--

gma-MIR1532

AACACGCUAAGCGAGAGGAGCUC

23

--

gma-MIR1533

AUAAUAAAAAUAAUAAUGA

19

--

gma-MIR1534

UAUUUUGGGUAAAUAGUCAU

20

--

gma-MIR1535

CUUGUUUGUGGUGAUGUCU

19

--

gma-MIR1536

AAGCAGAGACAAAUGUGUUUA

21

 

gma-MIR171b

CGAGCCGAAUCAAUAUCACUC

21

N (21 nt)

gma-MIR482

UUCCCAAUUCCGCCCAUUCCU

21

mi*

  1. a The most abundant read representing the family. Sequences with mismatches other than overhangs were marked as additional loci.
  2. b N denotes validation by Northern and the size of mature miRNA detected is indicated; mi* denotes validation by presence of miRNA* sequence in the library.
  3. cThe precursors of these miRNAs seems to be evolutionarily related, but the mature miRNAs are different from each other and we classified them as belonging to different families.