Figure 3

PARP-1 dependent genes in T cells involved in the immune response. (A) Expression maps including all the genes belonging to GO terms "immune response". Red indicates higher expression in Parp-1^+/+ T cells compared to Parp-1^-/- T cells (positive regulation by PARP-1) while green indicates higher expression in Parp-1^-/- T cells compared to Parp-1^+/+ T cells (negative regulation by PARP-1). Numbers in the left column indicate the fold-change expression in Parp-1^+/+ T cells compared to Parp-1^-/- T cells. (B) Real-time PCR analysis of representative genes in response to anti-CD3 stimulation or (C) in response to anti-CD3 + anti-CD28 stimulation. Samples were normalized according to Gapdh expression levels. The y-axis represents fold-change of activated versus resting cells for both Parp-1^+/+ (white bars) and Parp-1^-/- (black bars) T cells. Results represent the mean value ± SD of two independent experiments. The ratio of gene expression in Parp-1^+/+ over Parp-1^-/- cells (positive number) or the ratio of gene expression in Parp-1^-/- over Parp-1^+/+ (negative number) cells is indicated above each pair of bars.