Figure 4

Codon usage is predictive of expression level for individual genes. CAI scores were calculated for single-copy genes (see Fig. 3c and table 3 in the additional data file). The number of mass spectra, corrected for gene length, provides a measure of relative abundance. Only genes represented by ≥ 5 mass spectra from T. cruzi proteome analysis [21] were analysed to minimise sampling errors.