Figure 2

Evaluation of glioma-specific splicing events. (A) Plot of values extracted from Figure 1B examined by RT-PCR. False-negative samples had p-values > 0.05, but showed glioma-specific splicing by RT-PCR. False-positive samples had p-values < 0.05, but had no glioma-specific splicing by RT-PCR. Notable RefSeq entries are labeled with their gene names. The theoretical value for a 5-fold change in exon inclusion is shown (dashed line). For data on RefSeq entries with significant values see Additional file 6. (B) Representative RT-PCR validation results. The left panel shows RT-PCR results for nontumor brain (NB) and three GBM cell lines (GBM CL): U251 (1), SNB19 (2), and T98G (3). The right panel shows RT-PCR results for four nontumor brain samples and eight GBM tumor samples. The arrows indicate the isoform(s) that is differentially expressed in GBM; the involved exons are schematically presented to the right (NB, nontumor brain; GBM, GBM tumor brain). MBP and UBE2C were not observed to generate GBM-specific bands. GAPDH was used as a loading control. MW, molecular weight marker. For hybridization intensity maps for the highlighted genes see Additional file 7.