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Figure 4 | BMC Genomics

Figure 4

From: Sequence polymorphism can produce serious artefacts in real-time PCR assays: hard lessons from Pacific oysters

Figure 4

Outcomes of level of cDNA expression across families for primers (12, 13, 14) technically validated through the analysis of the melting curve of the EST [ AJ565694]. From left to right: Plot of PCR cycle number against logarithm PCR product amount; Derivative Melting Curve; Statistical results of level of cDNA expression across families based on a multiple comparison test (Tukey). Family numbers are reported on the horizontal axis, histograms sharing a letter are not significantly different (P < 0.05).

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BMC Genomics

ISSN: 1471-2164

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