Figure 5

Hybridization of the marker B76 with the Dra I-digested DNA of selected BAC clones and the wheat lines. Lanes 1–13: 1RS BAC clones showing some of the rye fragments missing in deletion mutants. Lane 14: Genomic DNA of Gabo1BL.1RS (wildtype; carrying the 1RS from cv. 'Imperial'); lane 15: interstitial deletion mutant (00.002); lane 16: mutant with chromosome arm loss (00.001). The capital letters (A to Q) show the position of rye fragments orthologous to the barley Mla resistance gene. The corresponding BAC fragments are shown as small letters. Arrows indicate the position of fragments (A, B & L) missing in the interstitial deletion mutant and maybe candidate for the stem rust resistance gene SrR. A detailed picture of the mutants with the loss of 1RS fragments is shown in Mago et al. (2004). All the samples were electrophoresed in the same gel however, two autoradiograms were developed. A 30 min exposure for the BAC clones and an overnight exposure to see the corresponding bands in the genomic DNA.