Figure 6

Quantitative RT-PCR of selected genes identified by microarray analysis. (A) Fold induction, as measured by quantitative RT-PCR, of the expression of representative genes identified as being enriched in CD41 positive cells (day 3.5) compared to VEGF-R2 positive, CD41 negative cells at day 2.5 (Table 1B). (B) Fold induction of representative genes in CD144 positive cells at day 3.5 (gray bars) and day 6.5 (black bars) compared to VEGF-R2 positive, CD144 negative cells at day 2.5 (Table 1C and D). (C) Fold induction of a subset of genes identified in CD144 positive versus CD144 negative cells in the developing mouse embryo at day E9.5, as measured by quantitative RT-PCR (Table 1E).