Figure 5

miR-14 expression across An. stephensi adult lifespan. Shown here are northern blots performed using Dig-labeled miRCURY LNA probes designed for hybridization to miR-14. The top panel is the northern result and the bottom panel is a corresponding RNA gel for verification of small ribosomal and tRNA integrity and equal loading of total RNA. ssDNA size markers (19 and 23 nts, not shown) were also visualized on the RNA gel for size estimation. Ten micrograms of total RNA for each sample were used. A) miR-14 expression in An. stephensi adult females fed with either sugar water (NBF, non-bloodfed) or blood meal (BF, bloodfed). The samples were 3, 5, 10, 17, and 24 day old adult females that were maintained on sugar water as well as adult females that were fed on blood on day 5 after emergence and collected at day 10, 17, and 24. Bloodfed females were allowed to oviposit two days after the blood meal. B) miR-14 expression in An. stephensi males and NBF females between 3–17 days of age. We did not extend the comparative analysis to 24 days post emergence because the majority of males do not survive that long.