Figure 1
From: Exploiting orthologue diversity for systematic detection of gain-of-function phenotypes
Schematic outline of the Xenoarray analysis procedure. An expression library from species A is transduced into target cells of species B. Efficient expression of the exogenous transcripts can be checked at this stage by comparing transduced and untransduced cells on microarrays for species A. Transduced cells are then subjected to a selective pressure to enrich for cells carrying exogenous cDNAs conferring resistance. Finally, comparing microarray signal intensities before and after selection allows simultaneous detection of all enriched exogenous transcripts, without the need of cloning resistant colonies to identify the integrated sequences.