Figure 4

A specific tryptic peptide observed in the TIGR annotation. In total, we identified 35 peptides which were specific to Sanger or TIGR datasets. This figure illustrates the only example observed only in the TIGR database. In (A), MS/MS information of the ion M+H = 1388.6600. While this MS/MS spectrum could not be identified by Mascot when using the Sanger database, it was identified as sequence HQQDYAALQGMK (inset with fragmentation pattern) only when the TIGR database was used. When the N-terminal region of this entry was aligned with the corresponding gene annotated by Sanger Rv3722 (B), it is clear that the Sanger entry (Top) failed to annotate the correct TSS for this gene. The identified sequence is underlined in the TIGR entry (bottom).