Cross-platform comparison of SYBR® Green real-time PCR with TaqMan PCR, microarrays and other gene expression measurement technologies evaluated in the MicroArray Quality Control (MAQC) study

Table 6 Comparison of the probing locations for the discordant genes between RT² Profiler PCR Arrays and other gene expression measurement systems in the MAQC study

	Gene Length (Bases)	RT² Profiler PCR Array	TaqMan	StaRT- PCR	QuantiGene	AFX	ABI	AG1	GEH	ILM
*FURIN*	4180	2060	4115	1382	2048	65	1	632	N/A	184
*RB1*	4740	4268	2068	1742	2053	46	171	119	1322	1471
*ABCD1*	3616	1139	2317	N/A	2292	104	565	314	355	102
*BAG1*	3858	3143	3319	2675	2750	148	3381	2597	2614	2718
*BAG4*	2182	941	1618	N/A	924	584	52	281	1774	802
*CDK5R1*	3870	2823	3597	N/A	2905	41	2948	2782	3403	689
*IGFBP5*	6316	4845	5198	5519	5074	411	4868	418	5882	4740
*JAK2*	5097	4761	1409	N/A	2216	36	612	1164	316	323

The most 3' interrogative locations of the above assays are indicated by the distance to the 3' end of the corresponding gene. The probing locations of the assays for technologies other than RT² Profiler PCR Arrays are available from the supplementary information provided by the MAQC study [8, 12]. Assays with discordant results compared to those of PCR Arrays are underlined and in bold.

ISSN: 1471-2164