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Table 1 Composition of the culture medium.

From: Growth rate regulated genes and their wide involvement in the Lactococcus lactis stress responses

Product

Concentration (g/L)

Product

Concentration (g/L)

Glucose

20

Isoleucine

0.025

Sodium acetate

1

Leucine

0.05875

Ammonium citrate

0.6

Valine

0.04125

KH2PO4

3 (9)

Cysteine

0.17

K2HPO4

2,5 (7,5)

Phenylalanine

0.28

MgCl2, 6H2O

0.2

Tyrosine

0.29

FeSO4, 7H2O

0.011

Adenine

0.05

CaCl2, 2H2O

0.05

Guanine

0.05

ZnSO4, 7H2O

0.005

Uracil

0.05

COCl2, 6H2O

0.0025

Xanthin

0.05

Alanine

0.24

P-aminobenzoic acid

0.01

Arginine

0.12

Biotin

0.01

Asparagine

0.34

Cyano-cobalamine (B12)

0.001

Glutamine

0.51

Folic acid

0.01

Glycine

0.17

Inosine

0.025

Histidine

0.11

Nicotinic acid

0.001

Lysine

0.35

Orotic acid

0.005

Methionine

0.12

Ca panthotenate

0.001

Proline

0.68

Pyridoxamine

0.005

Serine

0.34

Pyridoxin (B6)

0.002

Threonine

0.23

Riboflavin (B2)

0.001

Tryptophan

0.05

Thiamine

0.001

  

D, L-6,8-thioctic acid

0.0025

  

Thymidine

0.025

  1. The lines separating the different products indicate those that are dissolved together. Values into brackets in front of KH2PO4 and K2HPO4 indicate the required concentrations to obtain a buffered media for pre-cultures