PCR Primer Name | Primer Sequence | PCR Conditions |
---|---|---|
Prl6a1 forward | 5'-GGTCATTTCCCAGATGCTGT-3' | 1.5 mM MgCl2, 35 cycles, 60°C annealing |
Prl6a1 common reverse | 5'-GGGCTCCCTCCTTAGACACT-3' | Â |
Prl6a1 novel forward | 5'-GCCTGAGAAGGCAAAGAAAA-3 | Â |
Prl8a6 forward | 5'-CTGCCATTCAAGTCTCACGA-3' | 1.5 mM MgCl2, 30 cycles, 60°C annealing (Novel forward rxn – 35 cycles) |
Prl8a6 common reverse | 5'-CTCAAAAGCCAAGGAGATCG-3' | Â |
Prl8a6 novel forward | 5'-GCTCGATTATATAGGAACAGGAA-3' | Â |
Prl8a8 forward | 5'-GAAAAAGAATTGTGATT-3' | 2.0 mM MgCl2, 35 cycles, 46°C annealing (Novel forward rxn – 1.5 mM MgCl2) |
Prl8a8 common reverse | 5'-TCAATAGTTATGTATTGAA-3' | Â |
Prl8a8 novel forward | 5'-ATAACTCCGATGAAGC-3' | Â |
Prl8a1 reverse | 5'-CCAAACAATCAACTGCCATG-3' | 1.5 mM MgCl2, 30 cycles, 60°C annealing |
Prl8a1 common forward | 5'-AACCCCACTTCTGCACATTC-3' | Â |
Prl8a1 novel reverse | 5'-CCCAAAAGATCGACCCATAA-3' | Â |
Prl7a1 reverse | 5'-TCAGCCACATTTTCTGTTGC-3' | 2.0 mM MgCl2, 28 cycles, 60°C annealing (Novel reverse rxn 32 cycles) |
Prl7a1 common forward | 5'-AAGGGACTGTTGGATCATGC-3' | Â |
Prl7a1 novel reverse | 5'-TGGCATTCATGAGTGAGAAA-3' | Â |