Table 3 Strains and plasmids used in this study
From: Probing regulon of ArcA in Shewanella oneidensis MR-1 by integrated genomic analyses
Strain or plasmid | Description | Reference or source |
|---|---|---|
Strain or plasmid | Description | Reference or source |
   BL21 | F- omp T hsd SB(rB-mB-) gal dcm (DE3) | Invitrogen |
   WM3064 | Donor strain for conjugation; ΔdapA | 68 |
S. oneidensis strains | Â | Â |
   MR-1 | Wild-type | Lab stock |
   JZ3988K | arcA deletion mutant derived from MR-1; ΔarcA | This study |
   JZ3988K-CEM | JZ3988K with pBBR1MCS-5 | This study |
   JZ3988K-COM | JZ3988K with pBBR-ARCA | This study |
Plasmids | Â | Â |
   pDS3.1 | Apr, Gmr, derivative from suicide vector pCVD442 | 20 |
   pDS-ARCAK | pDS3.1 containing the PCR fragment for deleting arcA | This study |
   pBBR1MCS-5 | Gmr vector used for complementation, | 69 |
   pBBR-ARCA | pBBR1MCS-5 containing arcA and upstream promoter region from MR-1 | This study |
   pDONR221 | Entry vector of the Gateway system | Invitrogen |
   pDEST17 | Destination vector of the Gateway system (His-tag) | Invitrogen |