Development of genomic resources for Citrus clementina: Characterization of three deep-coverage BAC libraries and analysis of 46,000 BAC end sequences

Table 2 Citrus clementina BAC end sequencing summary

Library	CCL1	CCER1	CCH3	Total
Processed BACs	9,216	9,216	9,600	28,032
Positive BACs	8581	7262	8378	24,221
Success rate	93.1%	78.8%	87.3%	86.4%
BES mate pairs^a	8036	6401	7681	22,118
Genomic equivalents^b	3.1	3.1	3.9	10.1
Reads	16,617	13,663	16,059	46,339
Success rate	90%	74%	84%	83%
Total raw sequence (bp)	10,102,329	8,783,997	9,676,620	28,562,946
Average read length (bp)	638.0	679.6	644.3	652.8
Chloro/mito reads^c	432	146	204	782
Chloro/mito total sequence (bp)	273,452	94,570	111,324	479,346
Final genomic sequence (bp)^d	9,828,877	8,689,427	9,565,296	28,083,600

^a BAC clones with 5' and 3' good quality reads
^b Genomic equivalents calculated considering the number of clones sequenced and their average length
^c Reads identified as chloroplastic or mitochondrial DNA
^d Total genomic sequence obtained, without mitochondrial or chloroplastic DNA

ISSN: 1471-2164