Figure 4

Application of the adenoviral microarray to primary human cells (HUVEC). A. Percentile diagram showing the distribution of immunofluorescence intensity of HUVEC, indicative of lamin A/C: Cells were infected with adenovirus encoding either shCTR (open circles) or shLamin (closed circles) (p < 0.005; n > 150; by ANOVA analysis). B. Bar chart corresponding to the Lamin A/C expression levels shown in Fig. 4A: shCTR (white bars), shLamin (black bars) (p < 0.005; n > 150; by ANOVA analysis). C. Representative immunofluorescent images of HUVEC cells analysed in Figure 4D: Cells were infected with adenovirus encoding either shCTR (left), or shPBK (right). Green fluorescence (top) indicates infected cells, red fluorescence (middle) is indicative of activated caspase 3, merged images (bottom). Scale bar, 20 μm. D. Detection of apoptosis by immunostaining of α-cleaved caspase 3, using adenoviruses encoding specific shRNAs as indicated. The asterisks mark significant induction of apoptosis (4 spots, n > 150, p < 0.005; three independent experiments; by ANOVA analysis). TUNEL assay was not performed using HUVEC cells.