Figure 2

The loss of miR397 binding sites in a gene family. A: Phylogenetic tree of gene family of L-ascorbate oxidase precursor. The members predicted to be targets of miR397 are labelled with asterisks and the branch node where the WDG event occurred is indicated in blue bold line and the corresponding WGD gene pairs are shown in red lines. Os: Oryza sativa; At: Arabidopsis thaliana and Pt: Pinus taeda; B: The alignment of miR397 binding sites of four target genes from the WGD. The numbers of mismatch between miR397 and its binding sites are shown in parentheses. The mutation sites among the four genes are boxed.