Figure 3
From: Promoter-sharing by different genes in human genome – CPNE1 and RBM12 gene pair as an example
Reverse-Transcription PCR for the co-expression of RBM12 and CPNE1 in mouse tissues and human PBMC. A. Expression of the two genes in various mouse tissues. Top panel (mCPNE1): CPNE1 expression detected by CPNE1-specific primer pair; Second panel (mRBM12): PCR results from RBM12-specific primers; third panel(Com-mCPNE1): amplification from common forward primer from the non-coding exon2 and CPNE1-specific reverse primer from exon 4, the two bands reflect alternative splicing forms including/excluding exon 3; Lower panel(Com-mRBM12): PCR from common forward primer from non-coding exon2 and RBM12-specific primer from the RBM12-specific region of exon 3. B. Expression of the two genes in Human PBMC. Lane 1, 3, 5, 7, 9 are expression of CPNE1 from five individual blood donors, as amplified by a common forward primer in exon1 and CPNE1-specific reverse primer in exon 4. Lane 2, 4, 6, 8, and 10 are expression of RBM12 from the five individuals as amplified by common forward primer from exon 1 and a RBM12-specific primer in exon 3. The different bands in CPNE1 amplification reflect alternative splicing forms.