Table 1 Strains plasmids and primers used in this study
Strains | Â | Â |
|---|---|---|
Name | Genotype | Reference |
SL1344 | Parent strain | [73] |
SL1344 oxyR | SL1344 ΔoxyR | This work |
SL1344 fur | SL1344 Δfur | This work |
SL1344 sodA | SL1344 ΔsodA | This work |
SL1344 basS | SL1344 ΔbasS | This work |
SL1344pA | SL1344 (pMK1lux-PsodA) | This work |
SL1344pM | SL1344 (pMK1lux-PpmrH) | This work |
Plasmids | Â | Â |
Name | Description | Reference |
pBR322 | Cloning vector | [74] |
pSB377 | luxCDABE reporter operon | [75] |
pMK1 lux | pBR322 with luxCDABE operon and MCS | This work |
pMK1 lux -P sodA | pMK1lux with PsodA cloned as 5'EcoRI-3'BamHI fragment | This work |
pMK1 lux -P pmrH | pMK1lux with PpmrH cloned as 5'EcoRI-3'BamHI fragment | This work |
Primers | Â | Â |
Name | Sequence | Comment |
sodA5 | GCGGAATTC ATCAACAGGCG | cloning |
sodA3 | GCGGGATCC ATTATTGTCGAGC | cloning |
pmr5 | CGCGAATTC GCGAAATAGCGTTTG | cloning |
pmr3 | CGCGGATCC ATTGAAAGCCGCTTTTC | cloning |
pmr q5 | ATGTCGGACTTTTTGCCTTTC | qPCR |
pmr q3 | ATATTGATTGCCAGTTAGCC | qPCR |
sodA q5 | ATGAGTTATACACTGCCATC | qPCR |
sodA q3 | GCAAACTCAGGCAGGTTTTC | qPCR |
fhuA q5 | ATGGCGCGTCTTAAAACTGC | qPCR |
fhuA q3 | GCGGCAGGCGCTGCGGTTAC | qPCR |
invF q5 | ATGTCATTTTCTGAAAGCCG | qPCR |
invF q3 | AATGCCAGTAATTTGCTGAG | qPCR |
entE q5 | ATGCGTATACCTTTCACCCG | qPCR |
entE q3 | CTGAATGCGCGCTCGCCTTC | qPCR |
bas5 | CGCACGGTTCGCGGGTTTGG | λ-red |
bas3 | GTAGTGTGCTGATTGTCAGC | λ-red |
bas-P1 | CTACATGCTGGTTGCCACTGAGGAAAGCTAAGTGAGCCTGGTGTAGGCTGGAGCTGCTTC | λ-red |
bas-P4 | AGTTTTATCTATGTGTGGGTCACGACGTATTAAACGCCTGATTCCGGGGATCCGTCGACC | λ-red |
fur5 | AGTGCAATTTCTGTCACTTC | λ-red |
fur3 | CAGGAAAGAGGAGGATATAA | λ-red |
fur-P1 | TCTAATGAAGTGAATCGTTTAGCAACAGGACAGATTCCGCGTGTAGGCTGGAGCTGCTTC | λ-red |
fur-P4 | AAAAGCCAACCGGGCGGTTGGCTCTTCGAAAGATTTACACATTCCGGGGATCCGTCGACC | λ-red |
oxy5 | TAATCGTTCATTGCTATGCT | λ-red |
oxy3 | AACACCACCTTTAACTACCC | λ-red |
oxy-P1 | ACCTATCGCCATGAACTATCGTGGCGACGGAGGATGAATAGTGTAGGCTGGAGCTGCTTC | λ-red |
oxy-P4 | TCGGGTTGCGGCGTTGAACGGCTTAAACCGCCTGTTTTAAATTCCGGGGATCCGTCGACC | λ-red |