Adaptors (5'-3') | Primers (5'-3') | |
---|---|---|
Bsp1286I restriction site | ||
Preparation of genomic representations for genotyping | Bsp1286I adaptors I | Bsp1286I primer I |
• Forward strand: equimolar mix of CATAGGTGTCCACAGTCGGTCTGCA CATAGGTGTCCACAGTCGGTCTGCT CATAGGTGTCCACAGTCGGTCTGCC | AGGTGTCCACAGTCGGTCT | |
• Reverse strand GACCGACTGTGGAC | ||
Library construction | Bsp1286I adaptors II | Bsp1286I primer II |
• Forward strand: equimolar mix of CTGAGTAGTGCCAGAACGGTCTGCA CTGAGTAGTGCCAGAACGGTCTGCC CTGAGTAGTGCCAGAACGGTCTGCT | CTGAGTAGTGCCAGAACGGTC | |
• Reverse strand GACCGTTCTGGCA | ||
Pony sequence | - | 1536-clone preliminary library construction |
PonyAll primer | ||
CGNAATNTGARYCAAAACGGTA | ||
4608-clone expanded library construction and preparation of genomic representations for genotyping | ||
PonyB primer | ||
GGANGCGTATTCTTYACCCAC |