Table 2 Bacterial strains and plasmids
Name | Relevant genotype/informationa | Source/reference |
|---|---|---|
E. coli DH5αMCR | F- endA1 supE44 mcrA thi-1 hsdR17λ- recA1 relA1 Δ(lacZYA-argF) U169 (Φ80dlacZ ΔM15) gyrA96 deoR Δ(mrr-hsdRMS-mcrBC) | [58] |
ER2566 | F- λ- fhuA2 [lon] ompT lacZ::T7 gene1 gal sulA11 Δ(mcrC-mrr)114::IS10 R(mcr-73::miniTn10-TetS)2 R(zgb-210::Tn10)(TetS) endA1 [dcm] | New England Biolabs |
C. glutamicum | ||
ATCC 13032 | Wild type, NxR | ATCCb |
CR030 | ΔcysR | this study |
CR031 | mcbR ΔHTH ssuR ΔHTH cysR const | this study |
CR032 | mcbR ΔHTH ssuR ΔHTH cysR ΔHTH | this study |
CR031-035i | CR031 with integrated pCR035i, KmR | this study |
CR031-036i | CR031 with integrated pCR036i, KmR | this study |
CR031-037i | CR031 with integrated pCR037i, KmR | this study |
CR031-038i | CR031 with integrated pCR038i, KmR | this study |
CR031-039i | CR031 with integrated pCR039i, KmR | this study |
CR031-040i | CR031 with integrated pCR040i, KmR | this study |
CR031-041i | CR031 with integrated pCR041i, KmR | this study |
CR031-042i | CR031 with integrated pCR042i, KmR | this study |
CR031-043i | CR031 with integrated pCR043i, KmR | this study |
CR031-044i | CR031 with integrated pCR044i, KmR | this study |
CR031-045i | CR031 with integrated pCR045i, KmR | this study |
CR031-046i | CR031 with integrated pCR046i, KmR | this study |
CR031-047i | CR031 with integrated pCR047i, KmR | this study |
CR031-048i | CR031 with integrated pCR048i, KmR | this study |
CR032-035i | CR032 with integrated pCR035i, KmR | this study |
CR032-036i | CR032 with integrated pCR036i, KmR | this study |
CR032-037i | CR032 with integrated pCR037i, KmR | this study |
CR032-038i | CR032 with integrated pCR038i, KmR | this study |
CR032-039i | CR032 with integrated pCR039i, KmR | this study |
CR032-040i | CR032 with integrated pCR040i, KmR | this study |
CR032-041i | CR032 with integrated pCR041i, KmR | this study |
CR032-042i | CR032 with integrated pCR042i, KmR | this study |
CR032-043i | CR032 with integrated pCR043i, KmR | this study |
CR032-044i | CR032 with integrated pCR044i, KmR | this study |
CR032-045i | CR032 with integrated pCR045i, KmR | this study |
CR032-046i | CR032 with integrated pCR046i, KmR | this study |
CR032-047i | CR032 with integrated pCR047i, KmR | this study |
CR032-048i | CR032 with integrated pCR048i, KmR | this study |
Plasmids | ||
pK18mobsacB | sacB, lacZa, KmR, mcs | [56] |
pRIM2 | promoterless cat, KmR, dppc | [31] |
pTYB1 | ApR, carrying an intein-coupled chitin binding domain | New England Biolabs |
pCR030d | pK18mobsacB carrying cysR delc | this study |
pCR031d | pK18mobsacB carrying ssuR HTHdel | this study |
pCR032d | pK18mobsacB carrying cysR HTHdel | this study |
pCR033d | pK18mobsacB carrying mcbR HTHdel | this study |
pCR034d | pK18mobsacB carrying cysR constd | this study |
pCR035i | pRIM2 carrying PcysI-A | this study |
pCR036i | pRIM2 carrying PcysI-B | this study |
pCR037i | pRIM2 carrying PcysI-Am5 | this study |
pCR038i | pRIM2 carrying PcysI-Am3 | this study |
pCR039i | pRIM2 carrying Pfpr 2-A | this study |
pCR040i | pRIM2 carrying Pfpr 2-B | this study |
pCR041i | pRIM2 carrying PssuR-A | this study |
pCR042i | pRIM2 carrying PssuR-B | this study |
pCR043i | pRIM2 carrying Pcg 3372-A | this study |
pCR044i | pRIM2 carrying Pcg 3372-B | this study |
pCR045i | pRIM2 carrying Pcg 2810-A | this study |
pCR046i | pRIM2 carrying Pcg 2810-A1m | this study |
pCR047i | pRIM2 carrying Pcg 2810-A2m | this study |
pCR048i | pRIM2 carrying Pcg 2810-A1m2m | this study |
pCR050 | pTYB1 carrying a cysR-intein fusion with a C-terminal glycine | this study |