Figure 4

Real-time PCR verifies wt p53 binding detected by microarray analysis. The left panel displays the significance level of p53 binding to selected promoters according to the microarray data. The x-axis displays log(p-value) of enrichment over input. The vertical dashed line shows the p-value 0.01 which was used as a limit. The right panel depicts fold enrichment of p53 bound promoters as detected by chromatin immunoprecipitation and real-time PCR. The x-axis displays fold enrichment of immunoprecipitated sample over input. The vertical dashed line shows the level of the negative control gene GAPDH, which is not enriched. The genes that had significant increases in expression are marked with an asterisk.