Table 1 Standard oligonucleotide filters used in this work.
Oligonucleotide property | Condition for elimination |
|---|---|
Repeat sequences | Presence |
Constituent 20-mers | Non-uniqueness |
Homopolymers | Length > 5 |
Melting temperature | More than 5°C from median |
Self-folding energy | < -1 kcal/mol |
Similarity with other genomic location | > 70% |
15-mer count | > median |