Figure 2

Validation of genes reduced in the Foxa2 mutant by whole mount in situ hybridization. (A-C) Wild-type and (D-F) Foxa2 mutant embryos at E7.75. (A, D) Foxd4 expression is absent from node and AME, and reduced in the ANE in mutant embryos compared to wild-type. (B, E) Gal is completely absent from node, AME and PS in the mutant embryos compared to wild-type. (Note: the Foxa2 mutant embryo in (E) has some background signal due to over-staining to detect any residual Gal expression). (C, F) Itga3 is severely reduced in the mutant embryo compared to wild-type and is only expressed in a small area around the APS. Black arrowheads indicate node; black lines indicate AME; red arrowheads indicate ANE; black arrows indicate DE. Scale bars are 200 μm.