Figure 3

Genes we identified that are expressed in the node at E7.5. (A, B) Gal and (C, D) Pim1 are expressed in the PS and node. (E) Prnp, (F) Cyb561, (G) Smoc1, (I) 1700027A23Rik, (J) Mlf1, (K) 1700009P17Rik, and (L) Josd2 are all specifically expressed in the node (Note: later at E7.75, Smoc1 is expressed in the node and more broadly in the mesoderm; Additional File 3). (H) Gstm5 has widespread expression throughout the embryo, but is expressed strongly in the periphery of the node. (A, C, I) lateral view, anterior left; (B, D) distal view, anterior top; (E-H, J-L) anterior view.