Table 1 Sequence and assembly statistics
From: Analysis of the Pythium ultimum transcriptome using Sanger and Pyrosequencing approaches
Pyrosequencing ESTs | Sanger ESTs | Pyrosequencing + Sanger ESTs | Pyrosequencing assembly | Sanger assembly | Hybrid Assembly | |
|---|---|---|---|---|---|---|
No. sequences | 90,664 | 9,578 | 90,664 + 9,578 = 100,242 | 35,507 (11,155 assemblies) | 4,689 (1,584 assemblies) | 34,495 (10,811 assemblies) |
Total bases (bp) | 17,315,041 | 4,811,851 | 22,126,892 | 8,274,073 | 2,796,219 | 9,552,701 |
Minimum length (bp) | 100 | 100 | 100 | 100 | 100 | 100 |
Average length (bp) | 190 | 502 | 220 | 233 | 596 | 276 |
Maximum length (bp) | 425 | 1,002 | 1,002 | 1,385 | 4,283 | 4,471 |
%GC | 50.3 | 51.1 | 50.5 | 51.3 | 53.3 | 51.7 |
%N | 0.04 | 0 | 0.03 | 0.05 | 0 | 0.04 |