Table 4 PCR primer pairs used for genomic cloning of the different peach/almond allergen genes and for (bin) mapping the genes
From: Genomic characterization of putative allergen genes in peach/almond and their synteny with apple
Primers group | Reference Sequence1 | Primer name | Primer sequence (5' – 3') | Tm °C/cycles | Product | Gene | |
|---|---|---|---|---|---|---|---|
Pfu | Taq | (nt) | |||||
1 | DQ251187 | PpPr10f PpPr10r | ACCATGGGTGTCTTCACATA AATTTAGTTGTAGGCATCGG | 56/30 | 58/2 | 586/623 | Pru p/du 1.01/05 |
2 | DY646736 | Pp1-2f Pp1-2r | GTTTGTCCTTTAAACTCTCC GATTTAGTTGTAGGCATCGG | 53/30 | 55/2 | 762/765 | Pru p/du 1.02 |
3 | DY647300 | Pp1-3f Pp1-3r | CTTTGATCAGTTTCCCAAGT TTAGTTGTAGGCATCAGGGT | 53/30 | 55/2 | 624 | Pru p/du 1.03 |
4 | DY653062 | Pp1-4f1 Pp1-4r | TTTACAGAATCATGGGTGTG TTAGTTGTAGGCATCATGGT | 53/30 | 55/2 | 615/622 | Pru p/du 1.04 |
5 | GW1 | Pp1-1A-GSP1 Pp1-1A-GSP2 | AACCTTCACCAAAGCTAGTCTTCTTGATG GTCAGCATCCAGAACAAGGGCTTTGAACA | 1100 | Pru p/du 1.05 /6A/C | ||
6 | GW2 | P1-1A3-GSP1 P1-1A3-GSP2 | GGTGTCTTCACATACTCAGACGAGTC CCACCTCAGTCATCCCCCCACCAAGA | 1546 | Pru p/du 1.06A/C | ||
7 | P1-1-6 PpPr10r | CACCTCCAGTGCTCATAGC See above | 57/30 | 59/2 | 720 | Pru p/du 1.05 | |
8 | P1-7f P1-7r | ATGGGTGTCTTCACATACTCAG TTTAGTTGTAGGCATCTGGGT | 57/30 | 59/2 | 593 | Pru p/du 1.06A/B | |
9 | P1-8f P1-8r2 | TAGTTATGAGTTGCTTGCAATGCT GGGCATGCTTGCTTTGGTAACTC | 58/30 | 60/2 | 1346 | Pru p/du 1.06C | |
10 | P1-8f P1-9r | See above GAAAGTTCCAAAGTACATGTGC | 58/30 | 60/2 | 1908 | Pru p/du 1.06A | |
11 | AF362988 | PpTL2f PpTL2r | ACAGCAAGCCAATTAAGACA GCTTATGGGCAGAATGTGATGAG | 56/30 | 58/2 | 879/892 | Pru p/du 2.01A/B |
12 | AF362987 | PpTL1f PpTL1r | ATGATGAAGACCCTAGGAGCAG GTCGAGAGTCCTATCTTTAT | 56/30 | 58/2 | 974 | Pru p/du 2.02 |
13 | GW3 | PTLP1-GSP1 PTLP1-GSP2 | GATAATTGAGGATTTCCAAAGGAGGCTG AGGAGGGTTAAGCTGAGGCTGAGGACTG | 500/1500 | Pru p/du 2.02 | ||
14 | BF717226, DW349103 | PpTLP3f PpTLP3r | AAGTAAAGTTCTTTGGCGTC CACGTGGCACCACAAGCATC | 53/30 | 55/2 | 659 | Pru p/du 2.03 |
15 | GW4 | PruTLP3-GSP1 PruTLP3-GSP2 | AGTACTCACCCACCAACTTCTCCTGCGT GCTACTTTAGCCGAGTTCACTCTCAACG | 800/1000 | Pru p/du 2.03 | ||
16 | Consensus of 29 ESTs | PpTLP4f PpTLP4r | ACTTGTCTGAACTTATGGCTCC GAAGTTAGAAGAAGAGCTGCG | 58/30 | 60/2 | 1800/1825 | Pru p/du 2.04 |
17 | AY620230 X96714 | PpLTP1f1 PpLTP1r | ATCATAGTCAAGAGAGATGG CCCTAAGTGGATCACATAGC | 52/30 | 54/2 | 645 | Pru p/du 3.01 |
18 | AY093699 X96716 | PpLTP2f PpLTP2r | TATCAGCTTTACTTACGACG CTGGCTTCCACAGAAACCTC | 58/30 | 60/2 | 513/514 | Pru p/du 3.02 |
19 | X96715 | PpLTP3f PpLTP3r | CCCAAGCGAAAGAAACACTA CATCTCATATCATCCTTCCA | 58/30 | 60/2 | 527/528 | Pru p/du 3.03 |
20 | AJ491881 AY081852 | Pp4.01f Pp4.01r | AGAAGAAATCAGAAGCAACG AAATAGTCACTCGGAGCAAT | 51/30 | 53/2 | 1041 | Pru p/du 4.01 |
21 | AJ491882 | Pp4.02f Pp4.02r1 | CAGCAACAACAACAAAGATG TCTAGAGACCCTGCTCAACC | 53/30 | 55/2 | 754 | Pru p/du 4.02 |