New insights into the origin of the B genome of hexaploid wheat: Evolutionary relationships at the SPA genomic region with the S genome of the diploid relative Aegilops speltoides

Table 1 Conserved Coding (SPA gene) and Non-coding Sequences (CNS) identified between SPA-gA-gB-gD-gS at the 'SPA orthologous region'

Non coding 'SPA orthologous loci' sequences				Coding 'SPA orthologous loci' sequences
	B	D	S		B	D	S
A				A
CNS size (bp)	11 152	10 838	10 597	Nb of transitions	33	20	38
% Identity	85,9	87,9	86,8	Nb of transversions	22	10	23
Ks	0,874+- 0,036	1,037+-0,036	0,716+-0,024	Ratio	1,5	2	1,65
Ka	0,664+- 0,014	0,848+-0,015	0,57+-0,01	Ks	0,055+-0,015	0,042+-0,013	0,065+-0,016
Ks/Ka	1,3	1,2	1,3	Ka	0,042+-0,007	0,021+-0,005	0,049+-0,007
B				MYA	6,2–10,8	4,5–8,5	7,5–12,5
CNS size (bp)		10 666	11 976	B
% Identity		85,8	89,9	Nb de transitions		35	25
Ks		0,991+-0,034	0,617+-0,026	Nb de transversions		20	19
Ka		0,797+-0,015	0,492+-0,012	Ratio		1,75	1,32
Ks/Ka		1,2	1,3	Ks		0,071+-0,017	0,035+-0,012
D				Ka		0,039+-0,007	0,035+-0,006
CNS size (bp)			10 039	MYA		8,3–13,5	3,5–7,2
% Identity			85,3	D
Ks			0,902+-0,031	Nb de transitions			39
Ka			0,791+-0,014	Nb de transversions			23
Ks/Ka			1,1	Ratio			1,7
				Ks			0,089+-0,019
				Ka			0,043+-0,007
				MYA			10,8–16,6

Non-coding 'SPA orthologous loci' sequences. Detailed features obtained for the 6 pairwised alignments of the 4 SPA orthologous regions excluding the SPA gene itself are mentioned with the CNS length, percentage of sequence identity, Ks and Ka values and rate. As an example, alignment of the gA and gB SPA orthologous regions of respectively 31 498 bp and 18 589 bp correspond to a cumulative CNS length of 11 152 bp with 85.9% of sequence identity and 0.874, 0.664, 1.3 values for respectively Ks, Ka and Ks/Ka.
Coding 'SPA orthologous loci' sequences. 6 pairwised comparisons of the SPA gene between A/B B/D D/A A/S B/S D/S sequences are associated with the number of substitutions, the number of transition, the number of transversion, the transition/transversion ratio, the Ks value, the Ka value and speciation date (MYA).

ISSN: 1471-2164