Refined annotation and assembly of the Tetrahymena thermophila genome sequence through EST analysis, comparative genomic hybridization, and targeted gap closure

Table 2 Reannotated T. thermophila selenoprotein homologs.

Gene Type (Putative Selenoprotein)	Genbank ID	3' UTR Containing Putative SECIS (5'-3')	ESTs
Glutathione Peroxidase	TTHERM_00141160	AUUUUCAAAUAUUGAAAACUAAAAUGUUAAAUGAAAGAUUAUUUUUAAAUUUGUAAAAAAGAAAUAAUUUUGAAAAAAAUAUUAUUUUAGUUAGU	20 STV, 1 CNJ, 1 HVM, 1 MIN
Glutathione Peroxidase	TTHERM_00279820	GAUAAAAGAGAUAUCAUUCAAUGAUAGCUUUAUAAUUAAAUCUUUAAUAGAAGUUAUAAGGUUUGAAGCUAAUGAGCUCUAUUAUC	2 STV, 2 CNJ, 1 TSA
Thioredoxin Reductase	TTHERM_00486500	AAUUUAUAUAUCUUAAAGAUGUAUAGUAUAAUGAUAGCAAAUCUCGAAAAUCUUAGGAUUGGGAUUAGGCUUGAAUAGUCAGAGUAAUAAGAGUAUUUAUUA	0
Thioredoxin Reductase	TTHERM_00823430	UCUAAUAUGGAAAAUGACGAAUUUAGUCUAAACUGUAAAACAGGGAUUAAAUUCUGAA	6 STV, 1 TSA
Thioredoxin Reductase	TTHERM_00047660	AUACCUUCAACUGGUAGGAAUAUAAUGAUUAGAGAACUCCUAACCUCACUGAGGAGGGUUUUCUAUGAGGCAAGAUUAUUGAUUUUGUUGUAG	164 RCH, 11 STV, 5 CNJ, 1 HVM, 1 TSA, 1 MIN
Selenophosphate Synthetase 2	TTHERM_00522580	AAGUAUCAUUUCUAAAAUGAUGCAAAUUAUUACCUGAAACUCUAAAAGAGAAGGAAUUUGCUGAGAAAAAAAAUGAAAAUGAUAACUU	7 STV, 3 CNJ, 1 HVM, 1 TSA
Other Reannotated Genes
Glutathione Peroxidase	TTHERM_01099010	N.A.	0
Thioredoxin Reductase	TTHERM_00723630	N.A.	5 STV, 2 TSA
SelO	TTHERM_00852990	N.A.	4 STV

The first six are putative selenoproteins. EST library condition abbreviations are as in Table 1

ISSN: 1471-2164